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Literature summary extracted from

  • Norton, S.J.
    Purification and properties of the prolyl RNA synthetase of Escherichia coli (1964), Arch. Biochem. Biophys., 106, 147-152.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.15 3,4-Dehydroproline competitive inhibition of proline transfer to tRNA, no inhibition of proline-dependent ATP-diphosphate exchange Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 0.12
-
 Pro
-
 Escherichia coli
6.1.1.15 0.6
-
 ATP
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Escherichia coli
-
-
-
6.1.1.15 Escherichia coli 9723
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.15 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro 4-hydroxy-L-proline can replace proline in ATP-diphosphate exchange Escherichia coli AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Escherichia coli AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 4-hydroxy-L-proline can replace proline in ATP-diphosphate exchange Escherichia coli 9723 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro 3,4-dehydro-DL-proline can replace proline in ATP-diphosphate exchange Escherichia coli 9723 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.15 6.8 7.6 variation of the pH in this range has little effect on activity Escherichia coli