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Literature summary extracted from

  • Aoki, T.; Oya, H.
    Kinetic properties of carbamoyl-phosphate synthetase II (glutamine-hydrolyzing) in the parasitic protozoan Crithidia fasciculata and separation of the enzyme from aspartate carbamoyltransferase (1987), Comp. Biochem. Physiol. B, 87, 143-150.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 N-acetylglutamate no requirement for N-acetyl-L-glutamate Crithidia fasciculata

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 HCO3- 20 mM Crithidia fasciculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.5 0.1
-
L-Gln at 8.6% dimethyl sulfoxide plus 1.4% glycerol Crithidia fasciculata
6.3.5.5 0.14
-
L-Gln at 2.0% dimethyl sulfoxide plus 8.0% glycerol Crithidia fasciculata
6.3.5.5 0.27
-
L-Gln at 2.0% dimethyl sulfoxide plus 0.3% glycerol Crithidia fasciculata
6.3.5.5 1.2
-
HCO3- HCO3-, at 8.6% dimethyl sulfoxide plus 1.4% glycerol Crithidia fasciculata
6.3.5.5 1.7
-
ATP HCO3-, at 2.0% dimethyl sulfoxide plus 0.3% glycerol or 8.0% glycerol Crithidia fasciculata
6.3.5.5 1.7
-
ATP at 2.0% dimethyl sulfoxide plus 0.3% glycerol or 8.0% glycerol Crithidia fasciculata
6.3.5.5 26
-
NH4+ at 2.0% dimethyl sulfoxide plus 0.3% glycerol Crithidia fasciculata

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Crithidia fasciculata
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.5 0.00095
-
-
Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
Crithidia fasciculata 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
Crithidia fasciculata 2 ADP + phosphate + carbamoyl phosphate
-
?
6.3.5.5 ATP + L-glutamine + hydrogencarbonate + H2O ATP in form of MgATP2- Crithidia fasciculata ADP + phosphate + L-glutamate + carbamoyl phosphate
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.5.5 37
-
15 min, stable in absence of dimetyl sulfoxide and glycerol Crithidia fasciculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.5 6.7
-
highest activity in the pH-range 6.7-8.8 Crithidia fasciculata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.5.5 6.7 8.5 6.7: highest activity in the pH-range 6.7-8.5, pH 7.2: 95% of the activity at pH 6.7, pH 8.5: 64% of the activity at pH 6.7 Crithidia fasciculata