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Literature summary extracted from

  • He, Z.; Spain, J.C.
    A novel 2-aminomuconate deaminase in the nitrobenzene degradation pathway of Pseudomonas pseudoalcaligenes JS45 (1998), J. Bacteriol., 180, 2502-2506.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.99.5 CuSO4 2 mM, 20% inhibition Pseudomonas oleovorans
3.5.99.5 diethyldicarbonate 2 mM, complete inhibition Pseudomonas oleovorans
3.5.99.5 MnCl2 2 mM, 20% inhibition Pseudomonas oleovorans
3.5.99.5 additional information no inhibition by 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid Pseudomonas oleovorans
3.5.99.5 phenylhydrazine 10 mM, 20% inhibition Pseudomonas oleovorans
3.5.99.5 ZnCl2 2 mM, 70% inhibition Pseudomonas oleovorans
3.5.99.11 Cu2+ 2 mM, 20% inhibition Pseudomonas oleovorans
3.5.99.11 diethyl dicarbonate 2 mM, complete loss of activity Pseudomonas oleovorans
3.5.99.11 Mn2+ 2 mM, 20% inhibition Pseudomonas oleovorans
3.5.99.11 additional information not inhibitory: saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid, or EDTA at 25 mM Pseudomonas oleovorans
3.5.99.11 phenylhydrazine 10 mM, 80% inhibition Pseudomonas oleovorans
3.5.99.11 Zn2+ 2 mM, 70% inhibition Pseudomonas oleovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.99.5 0.067
-
 2-aminomuconate
-
 Pseudomonas oleovorans
3.5.99.11 0.067
-
 2-aminomuconate pH 6.6, 25°C Pseudomonas oleovorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.99.5 cytoplasm
-
 Pseudomonas oleovorans 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.99.5 additional information no requirement for divalent cations Pseudomonas oleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.5 16600
-
 6 * 16600, SDS-PAGE Pseudomonas oleovorans
3.5.99.5 100000
-
 gel filtration Pseudomonas oleovorans
3.5.99.11 16600
-
 6 * 16600, SDS-PAGE Pseudomonas oleovorans
3.5.99.11 100000
-
 gel filtration and PAGE Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.99.5 2-aminomuconate + H2O Pseudomonas oleovorans intermediate in biodegradation of nitrobenzene ?
-
 ?
3.5.99.5 2-aminomuconate + H2O Pseudomonas oleovorans JS45 intermediate in biodegradation of nitrobenzene ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.5 Pseudomonas oleovorans
-
 JS45
-
3.5.99.5 Pseudomonas oleovorans JS45
-
 JS45
-
3.5.99.11 Pseudomonas oleovorans P81593
-
-
3.5.99.11 Pseudomonas oleovorans JS45 P81593
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.5
-
 Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.99.5 37.8
-
-
 Pseudomonas oleovorans
3.5.99.11 37.8
-
 pH 6.6, 25°C Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.5 2-aminomuconate + H2O
-
 Pseudomonas oleovorans 4-oxalocrotonate + NH3
-
 ir
3.5.99.5 2-aminomuconate + H2O
-
 Pseudomonas oleovorans JS45 4-oxalocrotonate + NH3
-
 ir
3.5.99.5 2-aminomuconate + H2O intermediate in biodegradation of nitrobenzene Pseudomonas oleovorans ?
-
 ?
3.5.99.5 2-aminomuconate + H2O intermediate in biodegradation of nitrobenzene Pseudomonas oleovorans JS45 ?
-
 ?
3.5.99.5 additional information no activity with 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid Pseudomonas oleovorans ?
-
 ?
3.5.99.5 additional information no activity with 2-aminomuconic semialdehyde, glycine, alanine, aspartic acid, glutaric acid, 2-aminoadipic acid Pseudomonas oleovorans JS45 ?
-
 ?
3.5.99.11 2-aminomuconate + H2O the deamination reaction is irreversible under the assay conditions Pseudomonas oleovorans 4-oxalocrotonate + NH3 enzyme converts 2-aminomuconate directly to 4-oxalocrotonate, rather than 2-hydroxymuconate, which suggests that the deamination is carried out via an imine intermediate ir
3.5.99.11 2-aminomuconate + H2O the deamination reaction is irreversible under the assay conditions Pseudomonas oleovorans JS45 4-oxalocrotonate + NH3 enzyme converts 2-aminomuconate directly to 4-oxalocrotonate, rather than 2-hydroxymuconate, which suggests that the deamination is carried out via an imine intermediate ir
3.5.99.11 additional information no substrates: 2-aminomuconic semialdehyde, or saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid Pseudomonas oleovorans ?
-
 ?
3.5.99.11 additional information no substrates: 2-aminomuconic semialdehyde, or saturated alpha-amino acids, including glycine, alanine, aspartic acid, glutaric acid, and 2-aminoadipic acid Pseudomonas oleovorans JS45 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.99.5 hexamer 6 * 16600, SDS-PAGE Pseudomonas oleovorans
3.5.99.11 hexamer 6 * 16600, SDS-PAGE Pseudomonas oleovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.99.5 20
-
 stable for at least 3 days Pseudomonas oleovorans
3.5.99.5 60
-
 77% loss of activity in 5 min Pseudomonas oleovorans
3.5.99.11 22
-
 stable at room temperature for 3 days Pseudomonas oleovorans
3.5.99.11 60
-
 5 min, 23% residual activity Pseudomonas oleovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.99.5 208
-
 2-aminomuconate
-
 Pseudomonas oleovorans
3.5.99.11 208
-
 2-aminomuconate pH 6.6, 25°C Pseudomonas oleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.99.5 6.6
-
-
 Pseudomonas oleovorans
3.5.99.11 6.6
-
-
 Pseudomonas oleovorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.99.5 5.7 8.8 at room temperature for at least 3 h Pseudomonas oleovorans
3.5.99.11 5.7 8.8 room temperature, stable for at least 3 h Pseudomonas oleovorans