EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.10 | KCl | higher activity in buffers containing 50 mM | Homo sapiens | |
3.5.4.10 | NaCl | higher activity in buffers containing 150 mM | Homo sapiens |
EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.2.3 | medicine | - |
Gallus sp. |
2.1.2.3 | medicine | target for development of inhibitors with potential use as chemotherapeutic agents | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.3 | cloned and expressed | Salmonella enterica subsp. enterica serovar Typhimurium |
2.1.2.3 | cloned and expressed | Bacillus subtilis |
2.1.2.3 | cloned and expressed | Escherichia coli |
2.1.2.3 | purH gene cloning and sequencing of cDNA | Gallus sp. |
2.1.2.3 | purH gene, cloning and sequencing of cDNA, expressed in Escherichia coli | Homo sapiens |
3.5.4.10 | - |
Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.3 | 4-amino-10-methylpteroylglutamic acid | methotrexate, non-competitive inhibitor | Gallus sp. | |
2.1.2.3 | 4-amino-10-methylpteroylglutamic acid | methotrexate, non-competitive inhibitor | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.3 | 0.0168 | - |
5-aminoimidazole-4-carboxamide ribonucleotide | - |
Homo sapiens | |
2.1.2.3 | 0.0602 | - |
(6R,6S)-10-formyltetrahydrofolic acid | - |
Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.3 | 64425 | - |
1 * 64425, calculated from amino acid sequence | Homo sapiens |
2.1.2.3 | 64430 | - |
cDNA sequencing | Homo sapiens |
2.1.2.3 | 64450 | - |
electrospray mass spectroscopy | Homo sapiens |
2.1.2.3 | 65630 | - |
gel filtration | Homo sapiens |
3.5.4.10 | 64425 | - |
2 * 64425, open reading frame, polypeptide of 591 amino acids, 81% similar to chicken IMPCHase, truncation mutant, IMPCHase activity located within the NH2-terminal 223 amino acids | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | Salmonella enterica subsp. enterica serovar Typhimurium | - |
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | Bacillus subtilis | - |
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | Escherichia coli | - |
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | Gallus sp. | penultimate and final steps in the de novo purine biosynthesis pathway | tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | Homo sapiens | penultimate and final steps in the de novo purine biosynthesis pathway | tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
3.5.4.10 | 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | Homo sapiens | de novo purine synthesis step 10 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.3 | Bacillus subtilis | - |
- |
- |
2.1.2.3 | Escherichia coli | - |
- |
- |
2.1.2.3 | Gallus sp. | - |
avian | - |
2.1.2.3 | Homo sapiens | P31939 | human | - |
2.1.2.3 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
3.5.4.10 | Homo sapiens | P31939 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.3 | - |
Homo sapiens |
3.5.4.10 | near to homogeneity from Escherichia coli expression mutants | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.2.3 | CCRF-CEM cell | leukemia cell, T lymphoblastoid cell line | Homo sapiens | - |
2.1.2.3 | MCF-7 cell | breast cancer cell | Homo sapiens | - |
3.5.4.10 | hepatoma cell | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.2.3 | 1.19 | - |
- |
Homo sapiens |
3.5.4.10 | 2.8 | - |
extract from Escherichia coli expression mutants after several pufification steps | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.3 | (6R,6S)-10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide | - |
Homo sapiens | tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide | - |
r | |
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | - |
Bacillus subtilis | tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | - |
Escherichia coli | tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | penultimate and final steps in the de novo purine biosynthesis pathway | Gallus sp. | tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
2.1.2.3 | 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide | penultimate and final steps in the de novo purine biosynthesis pathway | Homo sapiens | tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide | - |
r | |
2.1.2.3 | additional information | in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
2.1.2.3 | additional information | in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene | Bacillus subtilis | ? | - |
? | |
2.1.2.3 | additional information | in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene | Escherichia coli | ? | - |
? | |
2.1.2.3 | additional information | in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene | Gallus sp. | ? | - |
? | |
2.1.2.3 | additional information | in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene | Homo sapiens | ? | - |
? | |
2.1.2.3 | N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide | - |
r | |
2.1.2.3 | N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide | - |
Bacillus subtilis | tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide | - |
r | |
2.1.2.3 | N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide | - |
Escherichia coli | tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide | - |
r | |
2.1.2.3 | N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide | - |
Gallus sp. | tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide | - |
r | |
2.1.2.3 | N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide | - |
Homo sapiens | tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide | - |
r | |
3.5.4.10 | 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | - |
Homo sapiens | IMP + H2O | - |
ir | |
3.5.4.10 | 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | de novo purine synthesis step 10 | Homo sapiens | ? | - |
? | |
3.5.4.10 | 5-formamidoimidazole-4-carboxamide | - |
Homo sapiens | hypoxanthine + H2O | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.3 | monomer | 1 * 64425, calculated from amino acid sequence | Homo sapiens |
3.5.4.10 | dimer | 2 * 64425, open reading frame, polypeptide of 591 amino acids, 81% similar to chicken IMPCHase, truncation mutant, IMPCHase activity located within the NH2-terminal 223 amino acids | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.2.3 | 5-aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolase | bifunctional enzyme | Homo sapiens |
2.1.2.3 | AICARFT | - |
Homo sapiens |
2.1.2.3 | AICARFT/IMPCHase | - |
Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.10 | 7.4 | - |
- |
Homo sapiens |