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Literature summary extracted from

  • Rayl, E.A.; Moroson, B.A.; Beardsley, G.P.
    The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping (1996), J. Biol. Chem., 271, 2225-2233.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.10 KCl higher activity in buffers containing 50 mM Homo sapiens
3.5.4.10 NaCl higher activity in buffers containing 150 mM Homo sapiens

Application

EC Number Application Comment Organism
2.1.2.3 medicine
-
Gallus sp.
2.1.2.3 medicine target for development of inhibitors with potential use as chemotherapeutic agents Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.3 cloned and expressed Salmonella enterica subsp. enterica serovar Typhimurium
2.1.2.3 cloned and expressed Bacillus subtilis
2.1.2.3 cloned and expressed Escherichia coli
2.1.2.3 purH gene cloning and sequencing of cDNA Gallus sp.
2.1.2.3 purH gene, cloning and sequencing of cDNA, expressed in Escherichia coli Homo sapiens
3.5.4.10
-
Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.3 4-amino-10-methylpteroylglutamic acid methotrexate, non-competitive inhibitor Gallus sp.
2.1.2.3 4-amino-10-methylpteroylglutamic acid methotrexate, non-competitive inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.3 0.0168
-
5-aminoimidazole-4-carboxamide ribonucleotide
-
Homo sapiens
2.1.2.3 0.0602
-
(6R,6S)-10-formyltetrahydrofolic acid
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.3 64425
-
1 * 64425, calculated from amino acid sequence Homo sapiens
2.1.2.3 64430
-
cDNA sequencing Homo sapiens
2.1.2.3 64450
-
electrospray mass spectroscopy Homo sapiens
2.1.2.3 65630
-
gel filtration Homo sapiens
3.5.4.10 64425
-
2 * 64425, open reading frame, polypeptide of 591 amino acids, 81% similar to chicken IMPCHase, truncation mutant, IMPCHase activity located within the NH2-terminal 223 amino acids Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Salmonella enterica subsp. enterica serovar Typhimurium
-
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Bacillus subtilis
-
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Escherichia coli
-
tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Gallus sp. penultimate and final steps in the de novo purine biosynthesis pathway tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Homo sapiens penultimate and final steps in the de novo purine biosynthesis pathway tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide Homo sapiens de novo purine synthesis step 10 ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.3 Bacillus subtilis
-
-
-
2.1.2.3 Escherichia coli
-
-
-
2.1.2.3 Gallus sp.
-
avian
-
2.1.2.3 Homo sapiens P31939 human
-
2.1.2.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
3.5.4.10 Homo sapiens P31939
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.3
-
Homo sapiens
3.5.4.10 near to homogeneity from Escherichia coli expression mutants Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.3 CCRF-CEM cell leukemia cell, T lymphoblastoid cell line Homo sapiens
-
2.1.2.3 MCF-7 cell breast cancer cell Homo sapiens
-
3.5.4.10 hepatoma cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.3 1.19
-
-
Homo sapiens
3.5.4.10 2.8
-
extract from Escherichia coli expression mutants after several pufification steps Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.3 (6R,6S)-10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
Homo sapiens tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
-
Salmonella enterica subsp. enterica serovar Typhimurium tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
-
Bacillus subtilis tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide
-
Escherichia coli tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide penultimate and final steps in the de novo purine biosynthesis pathway Gallus sp. tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
2.1.2.3 10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide penultimate and final steps in the de novo purine biosynthesis pathway Homo sapiens tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Bacillus subtilis ?
-
?
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Escherichia coli ?
-
?
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Gallus sp. ?
-
?
2.1.2.3 additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Homo sapiens ?
-
?
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
Salmonella enterica subsp. enterica serovar Typhimurium tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
r
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
Bacillus subtilis tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
r
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
Escherichia coli tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
r
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
Gallus sp. tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
r
2.1.2.3 N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
Homo sapiens tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
r
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide
-
Homo sapiens IMP + H2O
-
ir
3.5.4.10 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide de novo purine synthesis step 10 Homo sapiens ?
-
?
3.5.4.10 5-formamidoimidazole-4-carboxamide
-
Homo sapiens hypoxanthine + H2O
-
ir

Subunits

EC Number Subunits Comment Organism
2.1.2.3 monomer 1 * 64425, calculated from amino acid sequence Homo sapiens
3.5.4.10 dimer 2 * 64425, open reading frame, polypeptide of 591 amino acids, 81% similar to chicken IMPCHase, truncation mutant, IMPCHase activity located within the NH2-terminal 223 amino acids Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.2.3 5-aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolase bifunctional enzyme Homo sapiens
2.1.2.3 AICARFT
-
Homo sapiens
2.1.2.3 AICARFT/IMPCHase
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.10 7.4
-
-
Homo sapiens