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Literature summary extracted from
- Arginine catabolism in the phototrophic bacterium Rhodobacter capsulatus E1F1. Purification and properties of arginase (1992), J. Biochem., 204, 531-537.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.3.1 | putrescine | activating | Rhodobacter capsulatus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.3.1 | 2-mercaptoethanol | - |
Rhodobacter capsulatus | |
| 3.5.3.1 | Borate | - |
Rhodobacter capsulatus | |
| 3.5.3.1 | Cu2+ | - |
Rhodobacter capsulatus | |
| 3.5.3.1 | ethanol | - |
Rhodobacter capsulatus | |
| 3.5.3.1 | gamma-guanidinobutyrate | - |
Rhodobacter capsulatus | |
| 3.5.3.1 | L-ornithine | - |
Rhodobacter capsulatus | |
| 3.5.3.1 | p-hydroxymercuribenzoate | - |
Rhodobacter capsulatus | |
| 3.5.3.1 | Zn2+ | - |
Rhodobacter capsulatus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.3.1 | 16 | - |
L-Arg | - |
Rhodobacter capsulatus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.3.1 | Co2+ | activating | Rhodobacter capsulatus | |
| 3.5.3.1 | Fe2+ | slight activation | Rhodobacter capsulatus | |
| 3.5.3.1 | Mn2+ | activating | Rhodobacter capsulatus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.1 | 31000 | - |
4 * 31000, SDS-PAGE | Rhodobacter capsulatus |
| 3.5.3.1 | 117000 | - |
gel filtration and sucrose density gradient ultracentrifugation | Rhodobacter capsulatus |
| 3.5.3.1 | 126000 | - |
non-denaturing PAGE | Rhodobacter capsulatus |
| 3.5.3.1 | 138000 | - |
gel filtration | Rhodobacter capsulatus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.3.1 | Rhodobacter capsulatus | - |
E1F1 | - |
| 3.5.3.1 | Rhodobacter capsulatus E1F1 | - |
E1F1 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.1 | 183 | - |
- |
Rhodobacter capsulatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.3.1 | L-arginine + H2O | - |
Rhodobacter capsulatus | L-ornithine + urea | - |
? | |
| 3.5.3.1 | L-arginine + H2O | - |
Rhodobacter capsulatus E1F1 | L-ornithine + urea | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.3.1 | tetramer | 4 * 31000, SDS-PAGE | Rhodobacter capsulatus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.1 | -20 | - |
no loss of activity for several months | Rhodobacter capsulatus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.1 | 8.5 | 9.5 | - |
Rhodobacter capsulatus |
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