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Literature summary extracted from

  • Muro, T.; Tominaga, Y.; Okada, S.
    Purification and some properties of cyclo(Gly-Gly) hydrolase from a strain of Bacillus sp. No. 106 (1985), Agric. Biol. Chem., 49, 1567-1572.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.13 Cu2+
-
 Bacillus sp. (in: Bacteria)
3.5.2.13 monoiodoacetate
-
 Bacillus sp. (in: Bacteria)
3.5.2.13 Ni2+
-
 Bacillus sp. (in: Bacteria)
3.5.2.13 PCMB
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.13 11.1
-
 cyclo(Gly-Gly)
-
 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.2.13 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.13 Ca2+ activates Bacillus sp. (in: Bacteria)
3.5.2.13 Mg2+ activates Bacillus sp. (in: Bacteria)
3.5.2.13 Na+ activates Bacillus sp. (in: Bacteria)
3.5.2.13 Sr2+ activates Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.13 Bacillus sp. (in: Bacteria)
-
-
-
3.5.2.13 Bacillus sp. (in: Bacteria) No. 106
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.13 strain No. 106 Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.13 culture medium
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.13 additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.13 2,5-dioxopiperazine + H2O highly specific, does not hydrolyze other piperazines Bacillus sp. (in: Bacteria) glycylglycine
-
 ?
3.5.2.13 2,5-dioxopiperazine + H2O highly specific, does not hydrolyze other piperazines Bacillus sp. (in: Bacteria) No. 106 glycylglycine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.13 50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.13 30 70 30°C: about 50% of maximal activity, 70°C: about 70% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.13 50
-
 pH 7.0, 15 min, stable below Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.13 7.5 8
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.13 5 10 pH 5: about 20% of maximal activity, pH 10: about 50% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.13 5.5 9 30°C, 3 h, stable Bacillus sp. (in: Bacteria)