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Literature summary extracted from

  • Tang, T.Y.; Wen, C.J.
    Expression of the creatininase gene from Pseudomonas putida RS65 in Escherichia coli (2000), J. Ind. Microbiol., 24, 2-6.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.2.10 analysis analysis of serum creatinine concentration Pseudomonas putida
3.5.2.10 medicine diagnosis of renal, muscular and thyroid functions Alcaligenes sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.10 cloned, sequenced and expressed in Escherichia coli DH5?, vector pCRN741, creatininase gene consists of 771 bp, encodes 257 amino acids, similarity of RS65 to PS-7 72.8% and Arthrobacter sp. TE1826 43.2% Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.10 160000
-
-
 Alcaligenes sp.
3.5.2.10 224000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.10 Alcaligenes sp.
-
-
-
3.5.2.10 Pseudomonas putida
-
 NTU-8 and its creatininase producing mutant RS65
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.10 ammonium sulfate fractionation, DEAE Sepharose CL-6B and Sepharose CL-6B column chromatographies, 50% total activity Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.10 additional information
-
 by Escherichia coli transformants pCRN741 without and with IPTG 0.678 U per ml Pseudomonas putida

Subunits

EC Number Subunits Comment Organism
3.5.2.10 dimer dimer with a molecular weight of 175000 Alcaligenes sp.
3.5.2.10 octamer 8* 28000 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.10 45
-
-
 Pseudomonas putida
3.5.2.10 45
-
-
 Alcaligenes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.10 75
-
 30 min, activity still 40% Pseudomonas putida
3.5.2.10 75
-
 30 min, activity still 40% Alcaligenes sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.10 7 8
-
 Pseudomonas putida
3.5.2.10 7 8
-
 Alcaligenes sp.