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Literature summary extracted from

  • Ibrahim, S.A.; Lea, P.J.; Fowden, L.
    Purification and properties of 4-methyleneglutaminase from the leaves of peanut (Arachis hypogaea) (1984), Phytochemistry, 23, 1545-1549.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.67 additional information no activation by anions or carboxylic acids Arachis hypogaea

General Stability

EC Number General Stability Organism
3.5.1.67 glutamine stabilizes, protects against thermal denaturation Arachis hypogaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.67 4-methylene-L-glutamate non-competitive, Ki: 5.4 mM Arachis hypogaea
3.5.1.67 albizziine 20 mM: 50% inhibition Arachis hypogaea
3.5.1.67 arsenate very slight inhibition Arachis hypogaea
3.5.1.67 BO33- strong inhibition, non-competitive, Ki: 0.125 mM Arachis hypogaea
3.5.1.67 Cu2+ 1 mM: 20% inhibition Arachis hypogaea
3.5.1.67 D-glutamine 20 mM: 20% inhibition Arachis hypogaea
3.5.1.67 I- slight inhibition Arachis hypogaea
3.5.1.67 L-asparagine very slight inhibition, 20 mM: 3% inhibition Arachis hypogaea
3.5.1.67 L-glutamate non-competitive, Ki: 5.4 mM Arachis hypogaea
3.5.1.67 L-Glutamate-5-ethyl ester 20 mM: 40% inhibition Arachis hypogaea
3.5.1.67 L-glutamate-5-hydroxamate competitive, 20 mM: 35% inhibition, 4-methylene-L-glutamine as substrate Arachis hypogaea
3.5.1.67 L-glutamate-5-methyl ester 20 mM: 63% inhibition Arachis hypogaea
3.5.1.67 L-glutamate-diethyl ester 20 mM: 40% inhibition Arachis hypogaea
3.5.1.67 L-glutamine competitive, 20 mM: 50% inhibition, 4-methylene-L-glutamine as substrate Arachis hypogaea
3.5.1.67 L-N5-Ethylglutamine theanine, slight inhibition, 20 mM: 14% inhibition Arachis hypogaea
3.5.1.67 additional information insensitive to a variety of salts and carboxylic acids; kinetics of inhibition; no inhibition by phosphate, EDTA, a wide range of mono- and divalent cations, pyruvate, 2-oxoglutarate, SO42- Arachis hypogaea
3.5.1.67 N-acetyl-L-glutamate 20 mM: 78% inhibition Arachis hypogaea
3.5.1.67 N-acetyl-L-glutamine 20 mM: 29% inhibition Arachis hypogaea
3.5.1.67 NaBr slight inhibition Arachis hypogaea
3.5.1.67 NaCl slight inhibition Arachis hypogaea
3.5.1.67 NaCN very slight inhibition Arachis hypogaea
3.5.1.67 NaI slight inhibition Arachis hypogaea
3.5.1.67 Phenol red competitive, Ki: 3.8 mM Arachis hypogaea
3.5.1.67 S-Carbamoyl-L-cysteine 20 mM: 78% inhibition Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.67 additional information
-
 additional information kinetic data Arachis hypogaea
3.5.1.67 additional information
-
 additional information kinetics of inhibition Arachis hypogaea
3.5.1.67 13
-
 L-glutamine pH 7.2 Arachis hypogaea
3.5.1.67 13
-
 4-methylene-L-glutamine pH: 7.2 Arachis hypogaea
3.5.1.67 16
-
 L-glutamine pH 7.6 Arachis hypogaea
3.5.1.67 16
-
 4-methylene-L-glutamine pH: 7.6 Arachis hypogaea
3.5.1.67 20
-
 L-glutamate-5-hydroxamate
-
 Arachis hypogaea
3.5.1.67 20
-
 L-glutamine pH: 8.0 Arachis hypogaea
3.5.1.67 20
-
 4-methylene-L-glutamine pH: 8.0 Arachis hypogaea
3.5.1.67 27
-
 L-glutamine pH: 6.8 Arachis hypogaea
3.5.1.67 29
-
 4-methylene-L-glutamine pH: 6.8 Arachis hypogaea
3.5.1.67 36
-
 4-methylene-L-glutamine pH: 6.4 Arachis hypogaea
3.5.1.67 36
-
 L-glutamine pH 6.4 Arachis hypogaea
3.5.1.67 40
-
 L-glutamine pH 8.4 Arachis hypogaea
3.5.1.67 50
-
 4-methylene-L-glutamine pH: 8.4 Arachis hypogaea
3.5.1.67 67
-
 L-glutamine pH 6.0 Arachis hypogaea
3.5.1.67 67
-
 4-methylene-L-glutamine pH: 6.0 Arachis hypogaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.67 additional information metal ions not involved in catalytic reaction Arachis hypogaea
3.5.1.67 Na2HPO4 0.15 M, substrate 4-methylene-L-glutamine: very slight activation, substrate L-glutamine: no effect Arachis hypogaea
3.5.1.67 Na2SO4 0.15 M, substrate 4-methylene-L-glutamine: very slight activation, substrate L-glutamine: no effect Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.67 4-methylene-L-glutamine + H2O Arachis hypogaea
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.67 Arachis hypogaea
-
 peanut, groundnut
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.67
-
 Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.67 leaf
-
 Arachis hypogaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.67 0.042
-
-
 Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.67 4-methylene-L-glutamine + H2O
-
 Arachis hypogaea 4-methylene-L-glutamate + NH3
-
 ?
3.5.1.67 4-methylene-L-glutamine + H2O
-
 Arachis hypogaea ?
-
 ?
3.5.1.67 L-glutamate-5-ethyl ester + H2O about 1.6% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea ?
-
 ?
3.5.1.67 L-glutamate-5-hydroxamate + H2O about 6.6% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea ?
-
 ?
3.5.1.67 L-glutamate-5-methyl ester + H2O about 9% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea ?
-
 ?
3.5.1.67 L-glutamine + H2O about 20% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea L-glutamate + NH3
-
 ?
3.5.1.67 additional information substrate specificity Arachis hypogaea ?
-
 ?
3.5.1.67 additional information hydrolyses compounds with substituents on the amide group, e.g. esters and the hydroxamic acid derivative Arachis hypogaea ?
-
 ?
3.5.1.67 additional information L-N5-ethylglutamine, theanine, N-methyl-DL-glutamic acid, N-carbobenzoxy-L-glutamic acid, L-glutamic acid diethyl ester, N-acetyl-L-glutamine, D-glutamine, L-asparagine, O-carbamoyl-L-serine, S-carbamoyl-L-cysteine, albizziine, N-acetyl-L-glutamic acid: no substrates Arachis hypogaea ?
-
 ?
3.5.1.67 additional information L-asparagine: not a substrate Arachis hypogaea ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.67 37
-
 assay at Arachis hypogaea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.67 additional information
-
 glutamine protects against thermal denaturation Arachis hypogaea
3.5.1.67 40
-
 stable up to, above 40°C: activity lost rapidly, glutamine protects against thermal denaturation Arachis hypogaea
3.5.1.67 55
-
 10 min, about 50% loss of activity, glutamine protects against thermal denaturation Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.67 additional information
-
 activity sensitive to buffer composition Arachis hypogaea
3.5.1.67 8.5 11 broad plateau Arachis hypogaea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.67 3.8
-
 Phenol red competitive Arachis hypogaea
3.5.1.67 5.4
-
 L-glutamate non-competitive Arachis hypogaea
3.5.1.67 20
-
 albizziine
-
 Arachis hypogaea