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Literature summary extracted from

  • Traut, T.W.; Loechel, S.
    Pyrimidine catabolism: individual characterization of the three sequential enzymes with a new assay (1984), Biochemistry, 23, 2533-2539.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.2 0.0018
-
 Uracil
-
 Rattus norvegicus
1.3.1.2 0.011
-
 NADPH in presence of uracil Rattus norvegicus
3.5.1.6 0.0235
-
 N-Carbamoyl-beta-alanine
-
 Rattus rattus
3.5.2.2 0.011
-
 5,6-dihydrouracil
-
 Rattus norvegicus
3.5.2.2 117
-
 5,6-dihydrouracil
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.6 cytosol
-
 Rattus rattus 5829
-
3.5.2.2 cytosol
-
 Rattus norvegicus 5829
-
3.5.2.2 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.2 218000
-
 gel filtration Rattus norvegicus
3.5.1.6 234000
-
 gel filtration Rattus rattus
3.5.1.6 340000
-
 gel filtration Rattus rattus
3.5.2.2 140000
-
-
 Bos taurus
3.5.2.2 226000
-
-
 Bos taurus
3.5.2.2 226000
-
 gel filtration Rattus norvegicus
3.5.2.2 266000
-
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.6 N-carbamoyl-beta-alanine + H2O Mus musculus
-
 ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O Escherichia coli
-
 ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O Euglena gracilis
-
 ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O Rattus rattus
-
 ?
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Cavia porcellus
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Mus musculus
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Escherichia coli
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Bos taurus
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Oryctolagus cuniculus
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Euglena gracilis
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Canis lupus familiaris
-
 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O Cavia porcellus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Mus musculus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Escherichia coli
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Rattus norvegicus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Bos taurus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Oryctolagus cuniculus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Euglena gracilis
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Canis lupus familiaris
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Rattus norvegicus
-
-
-
3.5.1.6 Escherichia coli
-
-
-
3.5.1.6 Euglena gracilis
-
 plant-like unicellular flagellate
-
3.5.1.6 Mus musculus
-
 mouse
-
3.5.1.6 Rattus rattus
-
 rat
-
3.5.2.2 Bos taurus
-
 calf
-
3.5.2.2 Canis lupus familiaris
-
 dog
-
3.5.2.2 Cavia porcellus
-
 guinea-pig
-
3.5.2.2 Escherichia coli
-
-
-
3.5.2.2 Euglena gracilis
-
-
-
3.5.2.2 Mus musculus
-
 mouse
-
3.5.2.2 Oryctolagus cuniculus
-
 rabbit
-
3.5.2.2 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.2 liver
-
 Rattus norvegicus
-
3.5.1.6 hepatoma cell
-
 Rattus rattus
-
3.5.1.6 liver
-
 Mus musculus
-
3.5.1.6 liver
-
 Rattus rattus
-
3.5.2.2 hepatoma cell
-
 Rattus norvegicus
-
3.5.2.2 kidney
-
 Cavia porcellus
-
3.5.2.2 kidney
-
 Mus musculus
-
3.5.2.2 kidney
-
 Rattus norvegicus
-
3.5.2.2 kidney
-
 Bos taurus
-
3.5.2.2 kidney
-
 Oryctolagus cuniculus
-
3.5.2.2 kidney
-
 Canis lupus familiaris
-
3.5.2.2 liver
-
 Cavia porcellus
-
3.5.2.2 liver
-
 Mus musculus
-
3.5.2.2 liver
-
 Rattus norvegicus
-
3.5.2.2 liver
-
 Bos taurus
-
3.5.2.2 liver
-
 Oryctolagus cuniculus
-
3.5.2.2 liver
-
 Canis lupus familiaris
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.6 4°C, 50 mM Tris buffer with 10% glycerol + 10 mM 2-mercaptoethanol or 2 mM dithiothreitol enzyme loses less than 20% activity when maintained for 3 days Rattus rattus
3.5.2.2 -70°C, no loss of activity for several months Rattus norvegicus
3.5.2.2 4°C, less than 20% activity lost after 2-3 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.6 N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Mus musculus beta-alanine + CO2 + NH3
-
 ir
3.5.1.6 N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Escherichia coli beta-alanine + CO2 + NH3
-
 ir
3.5.1.6 N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Euglena gracilis beta-alanine + CO2 + NH3
-
 ir
3.5.1.6 N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Rattus rattus beta-alanine + CO2 + NH3
-
 ir
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Mus musculus ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Escherichia coli ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Euglena gracilis ?
-
 ?
3.5.1.6 N-carbamoyl-beta-alanine + H2O
-
 Rattus rattus ?
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Cavia porcellus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Mus musculus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Escherichia coli 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Rattus norvegicus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Bos taurus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Oryctolagus cuniculus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Euglena gracilis 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Canis lupus familiaris 3-ureidopropionate
-
 ?
3.5.2.2 5-fluoro-5,6-dihydrouracil + H2O less effective substrate Rattus norvegicus ?
-
 ?
3.5.2.2 hydantoin + H2O
-
 Cavia porcellus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Mus musculus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Escherichia coli N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Rattus norvegicus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bos taurus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Oryctolagus cuniculus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Euglena gracilis N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Canis lupus familiaris N-carbamoyl glycine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer
-
 Rattus norvegicus
3.5.2.2 tetramer
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 NADPH
-
 Rattus norvegicus