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Literature summary extracted from

  • Miyagawa, E.; Yano, J.; Hamakado, T.; Kido, Y.; Nishimoto, K.; Motoki, Y.
    Crystallization and properties of N-benzoylglycine amidohydrolase from Pseudomonas putida (1985), Agric. Biol. Chem., 49, 2881-2886.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.32 tetrahydral crystals Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.32 2,2'-dipyridyl 80% inhibition at 1 mM Pseudomonas putida
3.5.1.32 Ag2+ complete inhibition at 1 mM Pseudomonas putida
3.5.1.32 Ba2+ slight inhibition at 1 mM Pseudomonas putida
3.5.1.32 Co2+ slight inhibition at 1 mM Pseudomonas putida
3.5.1.32 Cu2+ complete inhibition at 1 mM Pseudomonas putida
3.5.1.32 Fe2+ 80% inhibition at 1 mM Pseudomonas putida
3.5.1.32 Fe3+ 42% inhibition at 1 mM Pseudomonas putida
3.5.1.32 Hg2+ complete inhibition at 1 mM Pseudomonas putida
3.5.1.32 iodoacetic acid 60% inhibition at 1 mM Pseudomonas putida
3.5.1.32 Mn2+ 60% inhibition at 1 mM Pseudomonas putida
3.5.1.32 Ni2+ complete inhibition at 1 mM Pseudomonas putida
3.5.1.32 o-phenanthroline complete inhibition at 1 mM Pseudomonas putida
3.5.1.32 p-chloromercuribenzoic acid complete inhibition at 1 mM Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.32 0.37
-
4-chloro-N-benzoylglycine
-
Pseudomonas putida
3.5.1.32 0.54
-
4-acetyl-N-benzoylglycine
-
Pseudomonas putida
3.5.1.32 0.54
-
N-[(4-hydroxyphenyl)carbonyl]glycine
-
Pseudomonas putida
3.5.1.32 0.71
-
4-chloro-N-benzoyl-L-alanine
-
Pseudomonas putida
3.5.1.32 0.72
-
hippurate
-
Pseudomonas putida
3.5.1.32 0.87
-
N-benzoyl-L-alanine
-
Pseudomonas putida
3.5.1.32 0.87
-
N-benzoyl-L-aminobutyric acid
-
Pseudomonas putida
3.5.1.32 0.95
-
4-hydroxy-N-benzoyl-L-alanine
-
Pseudomonas putida
3.5.1.32 1.1
-
4-acetyl-N-benzoyl-L-alanine
-
Pseudomonas putida
3.5.1.32 1.2
-
4-nitro-N-benzoylglycine
-
Pseudomonas putida
3.5.1.32 1.25
-
4-nitro-N-benzoyl-L-alanine
-
Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.32 42000
-
4 * 42000, SDS-PAGE Pseudomonas putida
3.5.1.32 170000
-
gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.32 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.32 to homogeneity, chromatography techniques Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.32 cell culture induction of enzyme synthesis by hippurate-containing media Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.32 426
-
-
Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.32 4-acetyl-N-benzoyl-L-alanine + H2O low activity Pseudomonas putida 4-acetylbenzoic acid + L-alanine
-
?
3.5.1.32 4-acetyl-N-benzoylglycine + H2O
-
Pseudomonas putida 4-acetylbenzoic acid + glycine
-
?
3.5.1.32 4-chloro-N-benzoyl-L-alanine + H2O
-
Pseudomonas putida 4-chlorobenzoic acid + L-alanine
-
?
3.5.1.32 4-chloro-N-benzoylglycine + H2O
-
Pseudomonas putida 4-chlorobenzoic acid + glycine
-
?
3.5.1.32 4-hydroxy-N-benzoyl-L-alanine low activity Pseudomonas putida 4-hydroxybenzoic acid + L-alanine
-
?
3.5.1.32 4-hydroxy-N-benzoylglycine + H2O
-
Pseudomonas putida 4-hydroxybenzoic acid + glycine
-
?
3.5.1.32 4-hydroxy-N-benzoylglycine-L-histidyl-L-leucine + H2O
-
Pseudomonas putida 4-hydroxybenzoic acid + L-histidyl-L-leucine
-
?
3.5.1.32 4-nitro-N-benzoyl-L-alanine + H2O
-
Pseudomonas putida 4-nitrobenzoic acid + L-alanine
-
?
3.5.1.32 4-nitro-N-benzoylglycine + H2O best substrate Pseudomonas putida 4-nitrobenzoic acid + glycine
-
?
3.5.1.32 N-(4-nitrobenzoyl)-Gly-Gly-Gly + H2O
-
Pseudomonas putida 4-nitrobenzoic acid + Gly-Gly-Gly
-
?
3.5.1.32 N-benzoyl-DL-methionine + H2O poor substrate Pseudomonas putida benzoic acid + DL-methionine
-
?
3.5.1.32 N-benzoyl-L-alanine + H2O
-
Pseudomonas putida benzoic acid + L-alanine
-
?
3.5.1.32 N-benzoyl-L-aminobutyric acid + H2O low activity Pseudomonas putida benzoic acid + L-aminobutyric acid
-
?
3.5.1.32 N-benzoyl-L-valine + H2O poor substrate Pseudomonas putida benzoic acid + L-valine
-
?
3.5.1.32 N-benzoylglycine + H2O hippurate Pseudomonas putida benzoic acid + glycine
-
?
3.5.1.32 N-benzoylglycylglycylglycine + H2O
-
Pseudomonas putida benzoic acid + glycylglycylglycine
-
?
3.5.1.32 N-[(4-hydroxyphenyl)carbonyl]glycine + H2O
-
Pseudomonas putida ?
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.32 tetramer 4 * 42000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.32 50
-
-
Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.32 50
-
stable up to Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.32 7 8
-
Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.32 6 8 stable between, labile in acidic pH Pseudomonas putida