BRENDA - Enzyme Database show

delta-Aminovaleramidase of Pseudomonas putida

Reitz, M.S.; Rodwell, V.W.; J. Biol. Chem. 245, 3091-3096 (1970)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.30
dithioerythritol
effectively reverses heavy metal inactivation
Pseudomonas putida
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.30
AgNO3
0.00125 mM, 100% inhibition
Pseudomonas putida
3.5.1.30
arsenite
characteristic of enzymes with essential vicinal sulfhydryl groups
Pseudomonas putida
3.5.1.30
Ba2+
5 mM, inhibition from 10-40%
Pseudomonas putida
3.5.1.30
Ca2+
5 mM, inhibition from 10-40%
Pseudomonas putida
3.5.1.30
Cd(acetate)2
0.00125 mM, 94% inhibition
Pseudomonas putida
3.5.1.30
Cd2+
0.5 mM, complete inhibition , characteristic of enzymes with essential vicinal sulfhydryl groups
Pseudomonas putida
3.5.1.30
Co2+
5.0 mM,75% inhibition
Pseudomonas putida
3.5.1.30
Cu2+
5.0 mM, complete inhibition
Pseudomonas putida
3.5.1.30
Fe2+
5 mM, inhibition from 10-40%
Pseudomonas putida
3.5.1.30
Hg(NO3)2
0.187 mM, 100% inhibition
Pseudomonas putida
3.5.1.30
Hg2+
0.5 mM, complete inhibition
Pseudomonas putida
3.5.1.30
HgCl2
0.00125 mM, 100% inhibition
Pseudomonas putida
3.5.1.30
Mg2+
5 mM, inhibition from 10-40%
Pseudomonas putida
3.5.1.30
NaAsO2
0.0125 mM, 47% inhibition
Pseudomonas putida
3.5.1.30
p-hydroxymercuribenzoate
0.00025 mM, 44% inhibition, inhibition partially reversed by dithioerythritol
Pseudomonas putida
3.5.1.30
Pb2+
5.0 mM, complete inhibition
Pseudomonas putida
3.5.1.30
Sn2+
5.0 mM, complete inhibition
Pseudomonas putida
3.5.1.30
Zn2+
0.5 mM, complete inhibition
Pseudomonas putida
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.30
12
-
6-Aminohexanamide
-
Pseudomonas putida
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.30
cytoplasm
-
Pseudomonas putida
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.30
Al3+
0.125 mM stimulates activity 37%
Pseudomonas putida
3.5.1.30
Fe3+
0.125 mM stimulates activity 25%
Pseudomonas putida
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.30
67000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.30
5-aminopentanamide + H2O
Pseudomonas putida
known intermediate in lysine catabolism
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.30
Pseudomonas putida
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.30
purified 400fold from the soluble fraction of cell-free extract
Pseudomonas putida
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.1.30
130
-
-
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.30
5-aminopentanamide + H2O
-
172031
Pseudomonas putida
5-aminopentanoate + NH3
-
-
-
?
3.5.1.30
5-aminopentanamide + H2O
known intermediate in lysine catabolism
172031
Pseudomonas putida
?
-
-
-
-
3.5.1.30
6-aminohexanamide + H2O
-
172031
Pseudomonas putida
6-aminohexanoate + NH3
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.30
7.5
8.5
-
Pseudomonas putida
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.30
7
9
stable below pH 6.5, irreversibly inactivated above pH 9
Pseudomonas putida
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.30
dithioerythritol
effectively reverses heavy metal inactivation
Pseudomonas putida
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.30
AgNO3
0.00125 mM, 100% inhibition
Pseudomonas putida
3.5.1.30
arsenite
characteristic of enzymes with essential vicinal sulfhydryl groups
Pseudomonas putida
3.5.1.30
Ba2+
5 mM, inhibition from 10-40%
Pseudomonas putida
3.5.1.30
Ca2+
5 mM, inhibition from 10-40%
Pseudomonas putida
3.5.1.30
Cd(acetate)2
0.00125 mM, 94% inhibition
Pseudomonas putida
3.5.1.30
Cd2+
0.5 mM, complete inhibition , characteristic of enzymes with essential vicinal sulfhydryl groups
Pseudomonas putida
3.5.1.30
Co2+
5.0 mM,75% inhibition
Pseudomonas putida
3.5.1.30
Cu2+
5.0 mM, complete inhibition
Pseudomonas putida
3.5.1.30
Fe2+
5 mM, inhibition from 10-40%
Pseudomonas putida
3.5.1.30
Hg(NO3)2
0.187 mM, 100% inhibition
Pseudomonas putida
3.5.1.30
Hg2+
0.5 mM, complete inhibition
Pseudomonas putida
3.5.1.30
HgCl2
0.00125 mM, 100% inhibition
Pseudomonas putida
3.5.1.30
Mg2+
5 mM, inhibition from 10-40%
Pseudomonas putida
3.5.1.30
NaAsO2
0.0125 mM, 47% inhibition
Pseudomonas putida
3.5.1.30
p-hydroxymercuribenzoate
0.00025 mM, 44% inhibition, inhibition partially reversed by dithioerythritol
Pseudomonas putida
3.5.1.30
Pb2+
5.0 mM, complete inhibition
Pseudomonas putida
3.5.1.30
Sn2+
5.0 mM, complete inhibition
Pseudomonas putida
3.5.1.30
Zn2+
0.5 mM, complete inhibition
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.30
12
-
6-Aminohexanamide
-
Pseudomonas putida
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.30
cytoplasm
-
Pseudomonas putida
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.30
Al3+
0.125 mM stimulates activity 37%
Pseudomonas putida
3.5.1.30
Fe3+
0.125 mM stimulates activity 25%
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.30
67000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.30
5-aminopentanamide + H2O
Pseudomonas putida
known intermediate in lysine catabolism
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.30
purified 400fold from the soluble fraction of cell-free extract
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.5.1.30
130
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.30
5-aminopentanamide + H2O
-
172031
Pseudomonas putida
5-aminopentanoate + NH3
-
-
-
?
3.5.1.30
5-aminopentanamide + H2O
known intermediate in lysine catabolism
172031
Pseudomonas putida
?
-
-
-
-
3.5.1.30
6-aminohexanamide + H2O
-
172031
Pseudomonas putida
6-aminohexanoate + NH3
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.30
7.5
8.5
-
Pseudomonas putida
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.30
7
9
stable below pH 6.5, irreversibly inactivated above pH 9
Pseudomonas putida