BRENDA - Enzyme Database

Enzymes of vitamin B6 degradation. Purification and properties of two N-acetylamidohydrolases

Huynh, M.S.; Snell, E.E.; J. Biol. Chem. 260, 2379-2383 (1985)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.5.1.29
stable at room temperature between pH 6 and 10
Pseudomonas sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.29
alpha-hydroxyglutarate
competitive inhibition
Pseudomonas sp.
3.5.1.29
alpha-ketoglutarate
-
Arthrobacter sp.
3.5.1.29
alpha-ketoglutarate
competitive inhibition
Pseudomonas sp.
3.5.1.29
citrate
competitive inhibition
Pseudomonas sp.
3.5.1.29
diphosphate
-
Arthrobacter sp.
3.5.1.29
diphosphate
competitive inhibition
Pseudomonas sp.
3.5.1.29
fumarate
-
Arthrobacter sp.
3.5.1.29
fumarate
competitive inhibition
Pseudomonas sp.
3.5.1.29
glutamate
competitive inhibition
Pseudomonas sp.
3.5.1.29
Glutarate
competitive inhibition
Pseudomonas sp.
3.5.1.29
Hg2+
-
Pseudomonas sp.
3.5.1.29
Itaconate
competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit
Arthrobacter sp.
3.5.1.29
Itaconate
competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit
Pseudomonas sp.
3.5.1.29
malate
competitive inhibition
Pseudomonas sp.
3.5.1.29
Maleate
competitive inhibition
Pseudomonas sp.
3.5.1.29
malonate
competitive inhibition
Pseudomonas sp.
3.5.1.29
N-acetyl-DL-aspartate
-
Arthrobacter sp.
3.5.1.29
N-acetyl-DL-aspartate
competitive inhibition
Pseudomonas sp.
3.5.1.29
oxaloacetate
-
Arthrobacter sp.
3.5.1.29
oxaloacetate
competitive inhibition
Pseudomonas sp.
3.5.1.29
phosphate
-
Arthrobacter sp.
3.5.1.29
phosphate
competitive inhibition
Pseudomonas sp.
3.5.1.29
SO32-
inhibitory effect increases rapidly with time to a constant level, not competitve with substrate
Arthrobacter sp.
3.5.1.29
SO32-
inhibitory effect increases rapidly with time to a constant level, not competitve with substrate
Pseudomonas sp.
3.5.1.29
succinate
-
Arthrobacter sp.
3.5.1.29
succinate
competitive inhibition
Pseudomonas sp.
3.5.1.66
Dicarboxylic acids
competitive
Arthrobacter sp.
3.5.1.66
diphosphate
competitive
Arthrobacter sp.
3.5.1.66
Hg2+
0.01 mM, 15 min, 82% activity remains
Arthrobacter sp.
3.5.1.66
Itaconic acid
competitive, most effective inhibitor, Ki: 0.74 mM
Arthrobacter sp.
3.5.1.66
malonate
competitive, Ki: 20 mM
Arthrobacter sp.
3.5.1.66
additional information
no inhibition by: organic protein reagents, 0.1 mM, 15 min, p-chloromercuribenzoate, 1.0 mM Ni2+, 1.0 mM Co2+, 1.0 mM Mn2+, 1.0 mM Mg2+, 1.0 mM Ca2+, 1.0 mM Cu2+, 1.0 mM Cs+, 1.0 mM Li+, SO42-, NO3-, Cl-, 10 mM dithiothreitol, 100 mM monocarboxylic acids
Arthrobacter sp.
3.5.1.66
phosphate
competitive
Arthrobacter sp.
3.5.1.66
sulfite
noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition
Arthrobacter sp.
3.5.1.66
Tricarboxylic acids
competitive
Arthrobacter sp.
3.5.1.66
Zn2+
1 mM: 40% inhibition
Arthrobacter sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.29
0.003
-
(N-acetylaminomethylene)succinic acid
at pH 6.5 and 25°C
Pseudomonas sp.
3.5.1.66
additional information
-
additional information
kinetic data
Arthrobacter sp.
3.5.1.66
0.025
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 25°C, Tris-HCl, pH 7.0
Arthrobacter sp.
3.5.1.66
0.123
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.0
Arthrobacter sp.
3.5.1.66
0.25
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, Tris-HCl, pH 8.0
Arthrobacter sp.
3.5.1.66
0.56
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.5
Arthrobacter sp.
3.5.1.66
0.68
-
3-(acetamidomethylene)succinate
compound A, 25°C, pH 7.0
Arthrobacter sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.66
additional information
some inorganic cations activate
Arthrobacter sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.29
32500
-
gel filtration
Pseudomonas sp.
3.5.1.66
36600
-
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
3.5.1.66
205000
-
Cr-7, gel filtration
Arthrobacter sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
Pseudomonas sp.
-
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
Pseudomonas sp. IA
-
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
involved in pyridoxine degradation
?
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp. IA
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
3.5.1.66
additional information
Pseudomonas sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
3.5.1.66
additional information
Arthrobacter sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
3.5.1.66
additional information
Pseudomonas sp. IA
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.29
Arthrobacter sp.
-
Cr-7
-
3.5.1.29
Pseudomonas sp.
-
MA-1
-
3.5.1.66
Arthrobacter sp.
-
Cr-7
-
3.5.1.66
Pseudomonas sp.
-
IA
-
3.5.1.66
Pseudomonas sp. IA
-
IA
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.29
-
Pseudomonas sp.
3.5.1.66
Cr-7
Arthrobacter sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.29
3.12
-
-
Pseudomonas sp.
3.5.1.66
additional information
-
-
Arthrobacter sp.
3.5.1.66
3.79
-
25°C
Arthrobacter sp.
Storage Stability
EC Number
Storage Stability
Organism
3.5.1.66
Cr-7, -20°C, 0.1 M Tris-HCl, pH 7.0, stable for several months
Arthrobacter sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp.
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
-
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp.
acetate + succinate semialdehyde + NH3 + CO2
same products as formed by acid hydrolysis
172028
Pseudomonas sp.
ir
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp. IA
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
-
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp. IA
acetate + succinate semialdehyde + NH3 + CO2
same products as formed by acid hydrolysis
172028
Pseudomonas sp. IA
ir
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Pseudomonas sp.
?
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Arthrobacter sp.
?
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Pseudomonas sp. IA
?
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
?
-
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
involved in pyridoxine degradation
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
?
-
-
-
-
3.5.1.66
3-(acetamidomethylene)succinate + H2O
compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate
172028
Arthrobacter sp.
acetate + 4-oxobutanoate + NH3 + CO2
-
172028
Arthrobacter sp.
?
3.5.1.66
additional information
high substrate specificity
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
additional information
enzyme highly specific, inducible amidohydrolase
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp.
?
-
-
-
-
3.5.1.66
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp. IA
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.29
monomer
-
Arthrobacter sp.
3.5.1.29
monomer
SDS-PAGE
Pseudomonas sp.
3.5.1.66
multimer
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.29
25
-
-
Pseudomonas sp.
3.5.1.66
25
-
assay at
Arthrobacter sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.29
70
90
at pH 8 and 70°C stable for 1 min, loses activity completely in 1 min at 90°C
Pseudomonas sp.
3.5.1.66
70
-
pH 8.0, 1 min, stable at
Arthrobacter sp.
3.5.1.66
100
-
1 min, 65% activity retained
Arthrobacter sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.29
1.67
-
(N-acetylaminomethylene)succinic acid
-
Pseudomonas sp.
3.5.1.66
2.3
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
turnover no./subunit, 25°C
Arthrobacter sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.29
6.5
7.5
pH 6.5 in 50 mM sodium phosphate, MES and HEPES buffers, pH 7.5 in Tris buffer
Pseudomonas sp.
3.5.1.66
additional information
-
pI: 4.2, effect of buffer on pH optimum
Arthrobacter sp.
3.5.1.66
6
7
-
Arthrobacter sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.29
6
10
most efficient between pH 6.0 and 7.0
Pseudomonas sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.29
6
10
loses activity rapidly between 4.0 and 5.0
Pseudomonas sp.
3.5.1.66
4
5
rapid loss of activity
Arthrobacter sp.
3.5.1.66
6
10
stable at room temperature
Arthrobacter sp.
3.5.1.66
8
-
70°C, 1 min, stable at
Arthrobacter sp.
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.66
0.74
-
Itaconic acid
competitive, most effective inhibitor
Arthrobacter sp.
3.5.1.66
20
-
malonate
competitive
Arthrobacter sp.
General Stability (protein specific)
EC Number
General Stability
Organism
3.5.1.29
stable at room temperature between pH 6 and 10
Pseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.29
alpha-hydroxyglutarate
competitive inhibition
Pseudomonas sp.
3.5.1.29
alpha-ketoglutarate
-
Arthrobacter sp.
3.5.1.29
alpha-ketoglutarate
competitive inhibition
Pseudomonas sp.
3.5.1.29
citrate
competitive inhibition
Pseudomonas sp.
3.5.1.29
diphosphate
-
Arthrobacter sp.
3.5.1.29
diphosphate
competitive inhibition
Pseudomonas sp.
3.5.1.29
fumarate
-
Arthrobacter sp.
3.5.1.29
fumarate
competitive inhibition
Pseudomonas sp.
3.5.1.29
glutamate
competitive inhibition
Pseudomonas sp.
3.5.1.29
Glutarate
competitive inhibition
Pseudomonas sp.
3.5.1.29
Hg2+
-
Pseudomonas sp.
3.5.1.29
Itaconate
competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit
Arthrobacter sp.
3.5.1.29
Itaconate
competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit
Pseudomonas sp.
3.5.1.29
malate
competitive inhibition
Pseudomonas sp.
3.5.1.29
Maleate
competitive inhibition
Pseudomonas sp.
3.5.1.29
malonate
competitive inhibition
Pseudomonas sp.
3.5.1.29
N-acetyl-DL-aspartate
-
Arthrobacter sp.
3.5.1.29
N-acetyl-DL-aspartate
competitive inhibition
Pseudomonas sp.
3.5.1.29
oxaloacetate
-
Arthrobacter sp.
3.5.1.29
oxaloacetate
competitive inhibition
Pseudomonas sp.
3.5.1.29
phosphate
-
Arthrobacter sp.
3.5.1.29
phosphate
competitive inhibition
Pseudomonas sp.
3.5.1.29
SO32-
inhibitory effect increases rapidly with time to a constant level, not competitve with substrate
Arthrobacter sp.
3.5.1.29
SO32-
inhibitory effect increases rapidly with time to a constant level, not competitve with substrate
Pseudomonas sp.
3.5.1.29
succinate
-
Arthrobacter sp.
3.5.1.29
succinate
competitive inhibition
Pseudomonas sp.
3.5.1.66
Dicarboxylic acids
competitive
Arthrobacter sp.
3.5.1.66
diphosphate
competitive
Arthrobacter sp.
3.5.1.66
Hg2+
0.01 mM, 15 min, 82% activity remains
Arthrobacter sp.
3.5.1.66
Itaconic acid
competitive, most effective inhibitor, Ki: 0.74 mM
Arthrobacter sp.
3.5.1.66
malonate
competitive, Ki: 20 mM
Arthrobacter sp.
3.5.1.66
additional information
no inhibition by: organic protein reagents, 0.1 mM, 15 min, p-chloromercuribenzoate, 1.0 mM Ni2+, 1.0 mM Co2+, 1.0 mM Mn2+, 1.0 mM Mg2+, 1.0 mM Ca2+, 1.0 mM Cu2+, 1.0 mM Cs+, 1.0 mM Li+, SO42-, NO3-, Cl-, 10 mM dithiothreitol, 100 mM monocarboxylic acids
Arthrobacter sp.
3.5.1.66
phosphate
competitive
Arthrobacter sp.
3.5.1.66
sulfite
noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition
Arthrobacter sp.
3.5.1.66
Tricarboxylic acids
competitive
Arthrobacter sp.
3.5.1.66
Zn2+
1 mM: 40% inhibition
Arthrobacter sp.
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.66
0.74
-
Itaconic acid
competitive, most effective inhibitor
Arthrobacter sp.
3.5.1.66
20
-
malonate
competitive
Arthrobacter sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.29
0.003
-
(N-acetylaminomethylene)succinic acid
at pH 6.5 and 25°C
Pseudomonas sp.
3.5.1.66
additional information
-
additional information
kinetic data
Arthrobacter sp.
3.5.1.66
0.025
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 25°C, Tris-HCl, pH 7.0
Arthrobacter sp.
3.5.1.66
0.123
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.0
Arthrobacter sp.
3.5.1.66
0.25
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, Tris-HCl, pH 8.0
Arthrobacter sp.
3.5.1.66
0.56
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.5
Arthrobacter sp.
3.5.1.66
0.68
-
3-(acetamidomethylene)succinate
compound A, 25°C, pH 7.0
Arthrobacter sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.66
additional information
some inorganic cations activate
Arthrobacter sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.29
32500
-
gel filtration
Pseudomonas sp.
3.5.1.66
36600
-
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
3.5.1.66
205000
-
Cr-7, gel filtration
Arthrobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
Pseudomonas sp.
-
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
Pseudomonas sp. IA
-
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Arthrobacter sp.
involved in pyridoxine degradation
?
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
Pseudomonas sp. IA
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
?
-
-
-
3.5.1.66
additional information
Pseudomonas sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
3.5.1.66
additional information
Arthrobacter sp.
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
3.5.1.66
additional information
Pseudomonas sp. IA
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.29
-
Pseudomonas sp.
3.5.1.66
Cr-7
Arthrobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.29
3.12
-
-
Pseudomonas sp.
3.5.1.66
additional information
-
-
Arthrobacter sp.
3.5.1.66
3.79
-
25°C
Arthrobacter sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.1.66
Cr-7, -20°C, 0.1 M Tris-HCl, pH 7.0, stable for several months
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp.
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
-
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp.
acetate + succinate semialdehyde + NH3 + CO2
same products as formed by acid hydrolysis
172028
Pseudomonas sp.
ir
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp. IA
acetate + succinate semialdehyde + NH3 + CO2
-
-
-
-
3.5.1.29
2-(acetamidomethylene)succinate + 2 H2O
-
172028
Pseudomonas sp. IA
acetate + succinate semialdehyde + NH3 + CO2
same products as formed by acid hydrolysis
172028
Pseudomonas sp. IA
ir
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Pseudomonas sp.
?
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Arthrobacter sp.
?
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
-
172028
Pseudomonas sp. IA
?
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp.
?
-
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
involved in pyridoxine degradation
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
172028
Pseudomonas sp. IA
?
-
-
-
-
3.5.1.66
3-(acetamidomethylene)succinate + H2O
compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate
172028
Arthrobacter sp.
acetate + 4-oxobutanoate + NH3 + CO2
-
172028
Arthrobacter sp.
?
3.5.1.66
additional information
high substrate specificity
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
additional information
enzyme highly specific, inducible amidohydrolase
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp.
?
-
-
-
-
3.5.1.66
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Arthrobacter sp.
?
-
-
-
-
3.5.1.66
additional information
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
172028
Pseudomonas sp. IA
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.29
monomer
-
Arthrobacter sp.
3.5.1.29
monomer
SDS-PAGE
Pseudomonas sp.
3.5.1.66
multimer
Cr-7, probably six identical subunits, 6 * 36600, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.29
25
-
-
Pseudomonas sp.
3.5.1.66
25
-
assay at
Arthrobacter sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.29
70
90
at pH 8 and 70°C stable for 1 min, loses activity completely in 1 min at 90°C
Pseudomonas sp.
3.5.1.66
70
-
pH 8.0, 1 min, stable at
Arthrobacter sp.
3.5.1.66
100
-
1 min, 65% activity retained
Arthrobacter sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.29
1.67
-
(N-acetylaminomethylene)succinic acid
-
Pseudomonas sp.
3.5.1.66
2.3
-
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
turnover no./subunit, 25°C
Arthrobacter sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.29
6.5
7.5
pH 6.5 in 50 mM sodium phosphate, MES and HEPES buffers, pH 7.5 in Tris buffer
Pseudomonas sp.
3.5.1.66
additional information
-
pI: 4.2, effect of buffer on pH optimum
Arthrobacter sp.
3.5.1.66
6
7
-
Arthrobacter sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.29
6
10
most efficient between pH 6.0 and 7.0
Pseudomonas sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.1.29
6
10
loses activity rapidly between 4.0 and 5.0
Pseudomonas sp.
3.5.1.66
4
5
rapid loss of activity
Arthrobacter sp.
3.5.1.66
6
10
stable at room temperature
Arthrobacter sp.
3.5.1.66
8
-
70°C, 1 min, stable at
Arthrobacter sp.