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Literature summary extracted from
- Biocytin-specific 110-kDa biotinidase from human serum (1993), Clin. Chim. Acta, 215, 63-71.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.12 | Hg2+ | - |
Homo sapiens |  |
| 3.5.1.12 | p-hydroxymercuribenzoate | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.12 | 0.00048 | - |
epsilon-N-biotinyl-L-lysine | 110000 Da biotinidase | Homo sapiens | |
| 3.5.1.12 | 0.0051 | - |
epsilon-N-biotinyl-L-lysine | 76000 Da biotinidase | Homo sapiens | |
| 3.5.1.12 | 0.009 | - |
p-(N-biotinylamino)benzoic acid | 110000 Da biotinidase | Homo sapiens | |
| 3.5.1.12 | 0.03 | - |
p-(N-biotinylamino)benzoic acid | 76000 Da biotinidase | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 76000 | - |
- |
Homo sapiens |
| 3.5.1.12 | 110000 | - |
gel filtration | Homo sapiens |
| 3.5.1.12 | 110000 | - |
isoenzyme, co-purified in addition to a 76000 kD biotinidase | Homo sapiens |
| 3.5.1.12 | 115000 | - |
- |
Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | biotin amide + H2O | Homo sapiens | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | Homo sapiens | - |
human | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.12 | 76000 Da and 110000 Da proteins copurified | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | blood | serum, plasma, leukocytes | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | biotin amide + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | ? | - |
? | |
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