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Literature summary extracted from

  • Garganta, C.L.; Wolf, B.
    Lipoamidase activity in human serum is due to biotinidase (1990), Clin. Chim. Acta, 189, 313-326.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.12 medicine plasma biotinidase activity assay for diagnosis and screen newborn infants for biotinidase deficiency, treatment of inborn metabolism errors with large doses of biotin Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.12 p-hydroxymercuribenzoate
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.12 92000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.12 biotin amide + H2O Homo sapiens
-
?
-
?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O Homo sapiens
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.12 Homo sapiens
-
human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.12
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.12 blood serum, plasma, leukocytes Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.12 -20°C, activity decreases upon storage Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.12 biotin amide + H2O
-
Homo sapiens ?
-
?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O
-
Homo sapiens biotin + L-lysine
-
ir
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O
-
Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.12 monomer 1 * 92000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.12 5 8 p-(N-biotinylamino)benzoic acid as substrate Homo sapiens