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Literature summary extracted from
- Reinigung und Eigenschaften der Biotinidase aus Schweinenieren und Lactobacillus casei (1963), Biochem. Z., 338, 599-613.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Enterococcus faecalis |  |
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Homo sapiens | |
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Lacticaseibacillus casei | |
| 3.5.1.12 | biotin | competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate | Sus scrofa | |
| 3.5.1.12 | diisopropylfluorophosphate | - |
Lacticaseibacillus casei | |
| 3.5.1.12 | diisopropylfluorophosphate | - |
Rattus rattus | |
| 3.5.1.12 | diisopropylfluorophosphate | - |
Sus scrofa | |
| 3.5.1.12 | iodoacetamide | - |
Homo sapiens | |
| 3.5.1.12 | iodoacetamide | - |
Sus scrofa | |
| 3.5.1.12 | p-hydroxymercuribenzoate | - |
Homo sapiens | |
| 3.5.1.12 | p-hydroxymercuribenzoate | - |
Rattus rattus | |
| 3.5.1.12 | p-hydroxymercuribenzoate | - |
Sus scrofa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.12 | 0.0042 | - |
p-(N-biotinylamino)benzoic acid | - |
Sus scrofa | |
| 3.5.1.12 | 0.016 | - |
epsilon-N-biotinyl-L-lysine | - |
Sus scrofa | |
| 3.5.1.12 | 0.02 | - |
p-(N-biotinylamino)benzoic acid | - |
Lacticaseibacillus casei | |
| 3.5.1.12 | 0.85 | - |
N-(1'-N-methoxycarbonyl-biotinyl)p-aminobenzoic acid | - |
Sus scrofa | |
| 3.5.1.12 | 5 | - |
1'-N-methoxycarbonyl-biocytin | - |
Sus scrofa | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | biotin amide + H2O | Homo sapiens | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Sus scrofa | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Enterococcus faecalis | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Lacticaseibacillus casei | - |
? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | Rattus rattus | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Homo sapiens | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Sus scrofa | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Enterococcus faecalis | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Lacticaseibacillus casei | - |
? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | Rattus rattus | - |
? | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.5.1.12 | urea | 50% activity in 3 M solution | Lacticaseibacillus casei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | Enterococcus faecalis | - |
- |
- |
| 3.5.1.12 | Homo sapiens | - |
human | - |
| 3.5.1.12 | Lacticaseibacillus casei | - |
- |
- |
| 3.5.1.12 | no activity in Achromobacter sp. | - |
- |
- |
| 3.5.1.12 | no activity in Lactobacillus arabinosus | - |
- |
- |
| 3.5.1.12 | Rattus rattus | - |
rat | - |
| 3.5.1.12 | Sus scrofa | - |
pig | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.12 | - |
Sus scrofa |
| 3.5.1.12 | - |
Lacticaseibacillus casei |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.12 | heart | - |
Sus scrofa | - |
| 3.5.1.12 | heart | - |
Rattus rattus | - |
| 3.5.1.12 | kidney | - |
Homo sapiens | - |
| 3.5.1.12 | kidney | - |
Sus scrofa | - |
| 3.5.1.12 | kidney | - |
Rattus rattus | - |
| 3.5.1.12 | liver | - |
Homo sapiens | - |
| 3.5.1.12 | liver | - |
Sus scrofa | - |
| 3.5.1.12 | liver | - |
Rattus rattus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 0.2 | 0.3 | - |
Sus scrofa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.12 | -20°C, mercaptoethanol, a few months without loss of activity | Sus scrofa |
| 3.5.1.12 | -20°C, phosphate buffer pH 7.5, a few months | Lacticaseibacillus casei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.12 | 1'-N-methoxycarbonyl-biocytin + H2O | - |
Sus scrofa | 1-N-methoxycarbonyl-biotin + L-lysine | - |
? | |
| 3.5.1.12 | 2-(N-biotinyl)-amino-5-nitro-benzoic acid + H2O | - |
Sus scrofa | biotin + 2-amino-5-nitrobenzoic acid | - |
? | |
| 3.5.1.12 | 2-(N-biotinyl)-amino-5-nitro-benzoic acid + H2O | - |
Lacticaseibacillus casei | biotin + 2-amino-5-nitrobenzoic acid | - |
? | |
| 3.5.1.12 | 3-(N-biotinyl)-amino-6-nitrobenzoic acid + H2O | - |
Sus scrofa | biotin + 3-amino-6-nitrobenzoic acid | - |
? | |
| 3.5.1.12 | 3-(N-biotinyl)-amino-6-nitrobenzoic acid + H2O | - |
Lacticaseibacillus casei | biotin + 3-amino-6-nitrobenzoic acid | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Lacticaseibacillus casei | ? | - |
? | |
| 3.5.1.12 | biotin amide + H2O | - |
Rattus rattus | ? | - |
? | |
| 3.5.1.12 | biotin methyl ester + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.5.1.12 | biotin methyl ester + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.5.1.12 | biotin methyl ester + H2O | - |
Lacticaseibacillus casei | ? | - |
? | |
| 3.5.1.12 | biotinyl-L-aspartate + H2O | - |
Sus scrofa | biotin + L-aspartate | - |
? | |
| 3.5.1.12 | biotinyl-L-aspartate + H2O | - |
Enterococcus faecalis | biotin + L-aspartate | - |
? | |
| 3.5.1.12 | biotinyl-L-aspartate + H2O | - |
Lacticaseibacillus casei | biotin + L-aspartate | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Lacticaseibacillus casei | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | biotin + L-lysine | - |
ir | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Enterococcus faecalis | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Lacticaseibacillus casei | ? | - |
? | |
| 3.5.1.12 | epsilon-N-biotinyl-L-lysine + H2O | - |
Rattus rattus | ? | - |
? | |
| 3.5.1.12 | N-(1'-N-methoxycarbonyl-biotinyl)p-aminobenzoic acid + H2O | - |
Sus scrofa | 1-N-methoxycarbonyl-biotin + 4-aminobenzoic acid | - |
? | |
| 3.5.1.12 | N-biotinyl-beta-alanine + H2O | - |
Sus scrofa | biotin + beta-alanine | - |
? | |
| 3.5.1.12 | N-biotinyl-beta-alanine + H2O | - |
Enterococcus faecalis | biotin + beta-alanine | - |
? | |
| 3.5.1.12 | N-biotinyl-beta-alanine + H2O | - |
Lacticaseibacillus casei | biotin + beta-alanine | - |
? | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Homo sapiens | biotin + p-aminobenzoic acid | - |
ir | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Sus scrofa | biotin + p-aminobenzoic acid | - |
ir | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Enterococcus faecalis | biotin + p-aminobenzoic acid | - |
ir | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Lacticaseibacillus casei | biotin + p-aminobenzoic acid | - |
ir | |
| 3.5.1.12 | p-(N-biotinylamino)benzoic acid + H2O | - |
Rattus rattus | biotin + p-aminobenzoic acid | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 6 | - |
- |
Sus scrofa |
| 3.5.1.12 | 6 | - |
- |
Rattus rattus |
| 3.5.1.12 | 7 | - |
- |
Lacticaseibacillus casei |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.12 | 4.5 | 7.5 | - |
Sus scrofa |
| 3.5.1.12 | 5.3 | 9.5 | - |
Lacticaseibacillus casei |
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