Any feedback?
Literature summary extracted from
- Purification and properties of ampicillin acylase from Pseudomonas melanogenum (1990), Biochim. Biophys. Acta, 1040, 12-18.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.11 | Cu2+ | decreases enzyme activity to 50% | Pseudomonas melanogenum |  |
| 3.5.1.11 | diethyldicarbonate | - |
Pseudomonas melanogenum | |
| 3.5.1.11 | Fe2+ | decreases enzyme activity to 50% | Pseudomonas melanogenum | |
| 3.5.1.11 | Hg2+ | decreases enzyme activity to 50% | Pseudomonas melanogenum | |
| 3.5.1.11 | N-bromosuccinimide | - |
Pseudomonas melanogenum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.11 | 1.61 | - |
cephalexin | - |
Pseudomonas melanogenum | |
| 3.5.1.11 | 1.79 | - |
cephadrine | - |
Pseudomonas melanogenum | |
| 3.5.1.11 | 1.96 | - |
cephaloglycin | - |
Pseudomonas melanogenum | |
| 3.5.1.11 | 4.34 | - |
ampicillin | - |
Pseudomonas melanogenum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.11 | intracellular | - |
Pseudomonas melanogenum | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 72000 | - |
2 * 72000, SDS-PAGE | Pseudomonas melanogenum |
| 3.5.1.11 | 146000 | - |
gel filtration | Pseudomonas melanogenum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | additional information | Pseudomonas melanogenum | other natural penicillins | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.11 | Pseudomonas melanogenum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.11 | - |
Pseudomonas melanogenum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | ampicillin + H2O | - |
Pseudomonas melanogenum | 6-aminopenicillanic acid + (R)-2-phenylglycine | - |
r | |
| 3.5.1.11 | cephadrine + H2O | - |
Pseudomonas melanogenum | ? | - |
? | |
| 3.5.1.11 | cephalexin + H2O | - |
Pseudomonas melanogenum | 7-amino-3-methyl-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid + D-phenylglycine | - |
? | |
| 3.5.1.11 | cephaloglycin + H2O | - |
Pseudomonas melanogenum | (6R,7R)-7-amino-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (2R)-amino(phenyl)acetic acid | - |
? | |
| 3.5.1.11 | additional information | other natural penicillins | Pseudomonas melanogenum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | dimer | 2 * 72000, SDS-PAGE | Pseudomonas melanogenum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 37 | - |
- |
Pseudomonas melanogenum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 6 | - |
- |
Pseudomonas melanogenum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
