EC Number | General Stability | Organism |
---|---|---|
3.5.1.11 | immobilized enzyme, higher stability than soluble enzyme | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.11 | 6-aminopenicillanic acid | non-competitive inhibition | Escherichia coli | |
3.5.1.11 | 6-aminopenicillanic acid | non-competitive inhibition | Priestia megaterium | |
3.5.1.11 | benzyl penicillin | - |
Escherichia coli | |
3.5.1.11 | phenylacetic acid | competitive inhibition | Escherichia coli | |
3.5.1.11 | phenylacetic acid | competitive inhibition | Priestia megaterium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.11 | 0.67 | - |
benzylpenicillin | - |
Escherichia coli | |
3.5.1.11 | 4.5 | - |
benzylpenicillin | - |
Priestia megaterium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.11 | extracellular | - |
Priestia megaterium | - |
- |
3.5.1.11 | intracellular | - |
Escherichia coli | 5622 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 70000 | - |
gel filtration | Escherichia coli |
3.5.1.11 | 71000 | - |
sedimentation equilibrium | Escherichia coli |
3.5.1.11 | 120000 | - |
sedimentation equilibrium | Priestia megaterium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.11 | Escherichia coli | - |
- |
- |
3.5.1.11 | Priestia megaterium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.11 | - |
Priestia megaterium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 48 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.11 | -20°C, freeze dried pure enzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.11 | 2-furylmethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + furan-2-yl-acetic acid | - |
? | |
3.5.1.11 | 2-thienylmethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + sulfo-(5-sulfothiophen-2-yl)-acetic acid | - |
? | |
3.5.1.11 | benzylpenicilloic acid + H2O | - |
Escherichia coli | ? | - |
? | |
3.5.1.11 | D-alpha-aminobenzylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + DL-alpha-aminophenylacetic acid | - |
? | |
3.5.1.11 | DL-alpha-hydroxybenzylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + DL-alpha-hydroxyphenylacetic acid | - |
? | |
3.5.1.11 | isobutoxymethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + carbonic acid isopropyl ester | - |
? | |
3.5.1.11 | additional information | overview on best substrates for similar organisms | Escherichia coli | ? | - |
? | |
3.5.1.11 | additional information | overview on best substrates for similar organisms | Priestia megaterium | ? | - |
? | |
3.5.1.11 | N-phenylacetyl-DL-leucine + H2O | - |
Escherichia coli | ? | - |
? | |
3.5.1.11 | N-phenylacetyl-L-alpha-aminophenylacetic acid + H2O | - |
Escherichia coli | ? | - |
? | |
3.5.1.11 | N-phenylacetylglycine + H2O | - |
Escherichia coli | ? | - |
? | |
3.5.1.11 | n-propoxymethylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + carbonic acid monopropyl ester | - |
? | |
3.5.1.11 | p-hydroxybenzylpenicillin + H2O | - |
Escherichia coli | 6-aminopenicillanate + p-hydroxyphenylacetic acid | - |
? | |
3.5.1.11 | penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanate + phenylacetic acid | - |
r | |
3.5.1.11 | penicillin G + H2O | - |
Priestia megaterium | 6-aminopenicillanate + phenylacetic acid | - |
? | |
3.5.1.11 | phenylacetamide + H2O | - |
Escherichia coli | ? | - |
? | |
3.5.1.11 | phenylacetamide + H2O | - |
Priestia megaterium | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | additional information | - |
overview on values for similar organisms | Escherichia coli |
3.5.1.11 | additional information | - |
overview on values for similar organisms | Priestia megaterium |
3.5.1.11 | 37 | - |
generally chosen because enzyme unstable at 45°C and 55°C | Escherichia coli |
3.5.1.11 | 37 | - |
generally chosen because enzyme unstable at 45°C and 55°C | Priestia megaterium |
3.5.1.11 | 45 | - |
benzylpenicillin | Priestia megaterium |
3.5.1.11 | 55 | - |
benzylpenicillin | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | additional information | - |
overview on values for similar organisms | Escherichia coli |
3.5.1.11 | additional information | - |
overview on values for similar organisms | Priestia megaterium |
3.5.1.11 | 8.2 | - |
- |
Escherichia coli |
3.5.1.11 | 8.5 | - |
- |
Priestia megaterium |