Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Duerre, J.A.
    A hydrolytic nucleosidase acting on S-adenosylhomocysteine and on 5'-methylthioadenosine (1962), J. Biol. Chem., 237, 3737-3741.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.2.9 unstable in solution of low ionic strength Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.9 5.4
-
 5'-methylthioadenosine
-
 Escherichia coli
3.2.2.9 9
-
 S-adenosylhomocysteine
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.9 5'-methylthioadenosine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O Escherichia coli
-
 ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O Klebsiella aerogenes
-
 ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O Escherichia coli B / ATCC 11303
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Escherichia coli
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Klebsiella aerogenes
-
 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O Escherichia coli B / ATCC 11303
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.9 Escherichia coli
-
-
-
3.2.2.9 Escherichia coli B / ATCC 11303
-
-
-
3.2.2.9 Klebsiella aerogenes
-
 NRRL No. 199
-
3.2.2.9 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.9 160-fold Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.9 3.75
-
 substrate S-adenosyl-L-homocysteine Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.2.2.9 0°C, loses 50% activity in 48 h, -20°C very unstable, lost nearly all activity when stored for 1 week Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.9 5'-methylthioadenosine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Escherichia coli 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Escherichia coli B / ATCC 11303 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Escherichia coli ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Klebsiella aerogenes ?
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Escherichia coli B / ATCC 11303 ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli B / ATCC 11303 adenine + S-ribosyl-L-homocysteine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Klebsiella aerogenes ?
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Escherichia coli B / ATCC 11303 ?
-
 ?
3.2.2.9 S-adenosylmethionine + H2O
-
 Klebsiella aerogenes adenine + hypoxanthine + methylthioribose + homoserine lactone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.9 nucleosidase
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.9 6.5
-
 optimal pH for both natural substrates, 5'-methylthioadenosine and S-adenosyl-L-homocysteine, with little variation from pH 5.7-7.1 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.9 4 8.5
-
 Escherichia coli