EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.14 | guanosine 3'-diphosphate-5'-triphosphate | guanine nucleotides, guanosinetriphosphate and polyphosphate derivatives, effective activation | Azotobacter vinelandii | |
3.2.2.14 | RNA | - |
Azotobacter vinelandii | |
3.2.2.14 | SO42- | 10 mM relative activity 103%, 100% activity with nicotinamide as substrate | Azotobacter vinelandii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.14 | 5-phosphoribose 1-diphosphate | PRPP, inorganic polyphosphates, affinity of 5-phosphoribose 1-pyrophosphate for the enzyme is dependent on the activator concentration , inhibition mechanism with 5-phosphoribose 1-pyrophosphate is competitive with GTP | Azotobacter vinelandii | |
3.2.2.14 | 7-methylguanosine-5'-triphosphate | - |
Azotobacter vinelandii | |
3.2.2.14 | ATP | - |
Azotobacter vinelandii | |
3.2.2.14 | CTP | - |
Azotobacter vinelandii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.14 | 0.0087 | - |
guanosine 3'-diphosphate 5'-triphosphate | - |
Azotobacter vinelandii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.2.14 | 110000 | - |
1 * 110000 + 1 * 110000, enzyme easily dissociates in presence of 1 mM ATP, consisting of 4 identical protomers, MW 55000, no dissociation in presence of GTP | Azotobacter vinelandii |
3.2.2.14 | 210000 | - |
gel filtration | Azotobacter vinelandii |
3.2.2.14 | 240000 | - |
- |
Azotobacter vinelandii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.14 | nicotinamide mononucleotide + H2O | Azotobacter vinelandii | biological function reusing NMN resulting from degradation of pyridine cofactors by nucleotide pyrophosphatase, EC 3.6.1.9, in concert with NMN aminohydrolase, EC 3.5.1.42 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.14 | Azotobacter vinelandii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.14 | - |
Azotobacter vinelandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.14 | guanosine 3'-diphosphate 5'-triphosphate + H2O | - |
Azotobacter vinelandii | ? | - |
? | |
3.2.2.14 | nicotinamide mononucleotide + H2O | biological function reusing NMN resulting from degradation of pyridine cofactors by nucleotide pyrophosphatase, EC 3.6.1.9, in concert with NMN aminohydrolase, EC 3.5.1.42 | Azotobacter vinelandii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.14 | dimer | 1 * 110000 + 1 * 110000, enzyme easily dissociates in presence of 1 mM ATP, consisting of 4 identical protomers, MW 55000, no dissociation in presence of GTP | Azotobacter vinelandii |