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Literature summary extracted from
- Purification and some properties of a protopectin-solubilizing enzyme that has potent activity on sugar beet protopectin (1990), Agric. Biol. Chem., 54, 879-889.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.99 | analysis | useful for studies on structure of protopectin | Bacillus subtilis |
| 3.2.1.99 | nutrition | useful for pectin production from sugar beet pulp | Bacillus subtilis |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.99 | IFO 3134, EDTA stabilizes during purification | Bacillus subtilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.99 | 2-mercaptoethanol | IFO 3134, slight inhibition | Bacillus subtilis |  |
| 3.2.1.99 | AgNO3 | IFO 3134 | Bacillus subtilis | |
| 3.2.1.99 | CdCl2 | IFO 3134 | Bacillus subtilis | |
| 3.2.1.99 | Fe2(SO4)3 | IFO 3134 | Bacillus subtilis | |
| 3.2.1.99 | Hg2+ | IFO 3134, completely | Bacillus subtilis | |
| 3.2.1.99 | HgCl2 | IFO 3134, completely | Bacillus subtilis | |
| 3.2.1.99 | Mn2+ | IFO 3134, completely | Bacillus subtilis | |
| 3.2.1.99 | MnCl2 | IFO 3134, completely | Bacillus subtilis | |
| 3.2.1.99 | additional information | - |
Bacillus subtilis | |
| 3.2.1.99 | NaN3 | IFO 3134, slight inhibition | Bacillus subtilis | |
| 3.2.1.99 | NaNO2 | IFO 3134, strong inhibition | Bacillus subtilis | |
| 3.2.1.99 | o-phenanthroline | IFO 3134, slight inhibition | Bacillus subtilis | |
| 3.2.1.99 | p-chloromercuribenzoate | IFO 3134, slight inhibition | Bacillus subtilis | |
| 3.2.1.99 | PbCl2 | IFO 3134 | Bacillus subtilis | |
| 3.2.1.99 | Sn2+ | IFO 3134, strong inhibition | Bacillus subtilis | |
| 3.2.1.99 | SnCl2 | IFO 3134, strong inhibition | Bacillus subtilis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.99 | BaCl2 | IFO 3134, slight activation | Bacillus subtilis | |
| 3.2.1.99 | CaCl2 | IFO 3134, slight activation | Bacillus subtilis | |
| 3.2.1.99 | CoCl2 | IFO 3134, very slight activation | Bacillus subtilis | |
| 3.2.1.99 | Hg2Cl2 | IFO 3134, slight activation | Bacillus subtilis | |
| 3.2.1.99 | MgCl2 | IFO 3134, activation | Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 27000 | - |
3134, gel filtration on Toyopearl HW-55S | Bacillus subtilis |
| 3.2.1.99 | 30000 | - |
IFO 3134, SDS-PAGE | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.99 | Bacillus subtilis | - |
IFO 3134 | - |
| 3.2.1.99 | Bacillus subtilis | - |
IFO 3022 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.99 | IFO 3134 | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 5.8 | - |
IFO 3134 | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.99 | 1,5-alpha-L-arabinan + H2O | - |
Bacillus subtilis | alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose | - |
? | |
| 3.2.1.99 | cell wall polysaccharide + H2O | - |
Bacillus subtilis | L-arabinan | - |
? | |
| 3.2.1.99 | L-arabinan + H2O | sugar beet pulp | Bacillus subtilis | L-arabino-oligosaccharides | - |
? | |
| 3.2.1.99 | additional information | IFO 3134, inactive on polygalacturonic acid and soluble pectin | Bacillus subtilis | ? | - |
? | |
| 3.2.1.99 | protopectin + H2O | IFO 3134, protopectin of sugar beet pulp and various citrus fruit peels, but much less on that from lemon peel | Bacillus subtilis | pectin | IFO 3134, highly polymerized pectin | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.99 | monomer | IFO 3134, 1 * 30000, SDS-PAGE | Bacillus subtilis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.99 | PPase-C | IFO 3134 | Bacillus subtilis |
| 3.2.1.99 | protopectinase C | IFO 3134 | Bacillus subtilis |
| 3.2.1.99 | protopectinase-C | IFO 3134 | Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 60 | - |
IFO 3134 | Bacillus subtilis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 20 | 80 | IFO 3134, activity between | Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 60 | - |
IFO 3134, up to, retains more than 80% of activity after 10 min | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 6 | - |
IFO 3134, at 37°C | Bacillus subtilis |
| 3.2.1.99 | 8 | - |
IFO 3134, at 60°C | Bacillus subtilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 4 | 10 | IFO 3134 | Bacillus subtilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 5 | 9 | IFO 3134, stable between at 50°C: 30 min | Bacillus subtilis |
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