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Literature summary extracted from

  • Nakakuki, T.; Azuma, K.; Kainuma, K.
    Action patterns of various exo-amylases and the anomeric configurations of their products (1984), Carbohydr. Res., 128, 297-310.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.98 extracellular
-
Klebsiella pneumoniae
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.98 Klebsiella pneumoniae
-
Aerobacter aerogenes
-
3.2.1.116 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.98
-
Klebsiella pneumoniae
3.2.1.116
-
Streptomyces griseus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.98 (Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose exo-alpha amylase, products have alpha-configuration Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.98 amylose + H2O short-chain amylose, DP, average degree of polymerization, = 23 Klebsiella pneumoniae maltohexaose main product, 40% maltohexaose ?
3.2.1.98 amylose + H2O short-chain amylose, DP, average degree of polymerization, = 23 Klebsiella pneumoniae maltohexaose alpha-configuration ?
3.2.1.98 amylose + H2O partially-oxidized potato amylose Klebsiella pneumoniae maltohexaose main product, 40% maltohexaose ?
3.2.1.98 amylose + H2O partially-oxidized potato amylose Klebsiella pneumoniae maltohexaose alpha-configuration ?
3.2.1.98 maltohexaose + H2O
-
Klebsiella pneumoniae maltose + maltotetraose main product ?
3.2.1.98 maltohexaose + H2O
-
Klebsiella pneumoniae D-glucose + maltopentaose
-
?
3.2.1.98 maltohexaose + H2O
-
Klebsiella pneumoniae maltotriose + ?
-
?
3.2.1.98 maltooligosaccharide + H2O
-
Klebsiella pneumoniae maltohexaose + ? alpha-configuration ?
3.2.1.98 additional information products have alpha-configuration Klebsiella pneumoniae ?
-
?
3.2.1.98 starch + H2O blue starch: not a substrate Klebsiella pneumoniae maltohexaose + ?
-
?
3.2.1.116 amylose + H2O partially oxidized potato amylose Streptomyces griseus maltotriose
-
?
3.2.1.116 maltooligosaccharide + H2O enzyme hydrolyzes maltooligosaccharides larger than maltotriose by an exo-mechanism from the nonreducing end Streptomyces griseus maltotriose alpha-configuration ?
3.2.1.116 short-chain amylose + H2O
-
Streptomyces griseus maltotriose alpha-configuration ?
3.2.1.116 short-chain amylose + H2O
-
Streptomyces griseus maltotriose major initial product, prolonged action leads to gradual hydrolysis of maltotriose to glucose and maltose ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.98 Aerobacter aerogenes amylase
-
Klebsiella pneumoniae
3.2.1.98 G6-forming amylase
-
Klebsiella pneumoniae
3.2.1.98 G6-forming exo-alpha amylase
-
Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.116 40
-
assay at Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.116 7
-
assay at Streptomyces griseus