EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.8 | expression in Escherichia coli | Caldicellulosiruptor saccharolyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | iodoacetate | 5 mM, 21% loss of activity | Caldicellulosiruptor saccharolyticus | |
3.2.1.8 | Ni2+ | 5 mM, complete inhibition | Caldicellulosiruptor saccharolyticus | |
3.2.1.8 | RbCl | 5 mM, 55% inhibition | Caldicellulosiruptor saccharolyticus | |
3.2.1.8 | SDS | 1% w/w, 86% loss of activity | Caldicellulosiruptor saccharolyticus | |
3.2.1.8 | xylose | no inhibition | Caldicellulosiruptor saccharolyticus | |
3.2.1.8 | Zn2+ | 5 mM, complete inhibition | Caldicellulosiruptor saccharolyticus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | K+ | 200 mM, slight activation | Caldicellulosiruptor saccharolyticus | |
3.2.1.8 | Li+ | 200 mM, slight activation | Caldicellulosiruptor saccharolyticus | |
3.2.1.8 | Mg2+ | 5 mM MgCl2, slight activation | Caldicellulosiruptor saccharolyticus | |
3.2.1.8 | Na+ | 200 mM NaCl, slight activation | Caldicellulosiruptor saccharolyticus | |
3.2.1.8 | SrCl2 | 5 mM, slight activation | Caldicellulosiruptor saccharolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 32000 | - |
gel filtration | Caldicellulosiruptor saccharolyticus |
3.2.1.8 | 42000 | - |
1 * 42000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Caldicellulosiruptor saccharolyticus | - |
- |
- |
3.2.1.8 | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.8 | recombinant enzyme expressed in Escherichia coli | Caldicellulosiruptor saccharolyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 167 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | 1,4-beta-D-xylan + H2O | low activity on larchwood xylan | Caldicellulosiruptor saccharolyticus | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
3.2.1.8 | 1,4-beta-D-xylan + H2O | larchwood xylan | Caldicellulosiruptor saccharolyticus | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
3.2.1.8 | 1,4-beta-D-xylan + H2O | oat spelt xylan | Caldicellulosiruptor saccharolyticus | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
3.2.1.8 | 1,4-beta-D-xylan + H2O | low activity on larchwood xylan | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
3.2.1.8 | 1,4-beta-D-xylan + H2O | larchwood xylan | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
3.2.1.8 | 1,4-beta-D-xylan + H2O | oat spelt xylan | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | additional information | release of xylobiose and some xylotriose from oat spelt xylan | ? | |
3.2.1.8 | 4-methylumbelliferyl beta-D-cellobioside + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.8 | acetylhemicellulose + H2O | high activity on sugar cane bagasse hemicellulose A and B | Caldicellulosiruptor saccharolyticus | hemicellulose + acetate | - |
? | |
3.2.1.8 | acetylhemicellulose + H2O | high activity on sugar cane bagasse hemicellulose A and B | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | hemicellulose + acetate | - |
? | |
3.2.1.8 | carboxymethylcellulose + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.8 | p-nitrophenyl beta-D-cellobioside + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.8 | p-nitrophenyl beta-D-xylopyranoside | transferase activity on | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.8 | xylobiose + H2O | no hydrolysis | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.8 | xylobiose + H2O | no hydrolysis | Caldicellulosiruptor saccharolyticus Tp8T6.3.3.1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.8 | monomer | 1 * 42000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 70 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 40 | 80 | 40°C: about 30% of maximal activity, 80°C: about 20% of maximal activity | Caldicellulosiruptor saccharolyticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 70 | - |
pH 6.0, half-life: 4 min in absence of added protein, 30 min, in presence of 0.5 mg/ml bovine serum albumin | Caldicellulosiruptor saccharolyticus |
3.2.1.8 | 80 | - |
pH 6.0, half-life is 20 min in presence of 0.5 mg/ml bovine serum albumin | Caldicellulosiruptor saccharolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 6.5 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 5 | 7.7 | more than 50% of maximal activity at pH 5.0 and at pH 7.0 | Caldicellulosiruptor saccharolyticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 4.5 | 11.5 | stable at room temperature in presence of 0.5 mg/ml bovine serum albumin | Caldicellulosiruptor saccharolyticus |