Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Khasin, A.; Alchanati, I.; Shoham, Y.
    Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6 (1993), Appl. Environ. Microbiol., 59, 1725-1730.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.8 paper production bleaching of pulp Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ 1 mM, 52% inhibition Geobacillus stearothermophilus
3.2.1.8 Ag2+ 1 mM Ag(NO3)2, 33% inhibition Geobacillus stearothermophilus
3.2.1.8 Al3+ 1 mM, 46% inhibition Geobacillus stearothermophilus
3.2.1.8 Cd2+
-
 Geobacillus stearothermophilus
3.2.1.8 Co2+ 1 mM, 22% inhibition Geobacillus stearothermophilus
3.2.1.8 Fe2+ 10 mM, 37% inhibition Geobacillus stearothermophilus
3.2.1.8 Hg2+ 0.1 mM, 30% inhibition Geobacillus stearothermophilus
3.2.1.8 Mn2+ 1 mM, 38% inhibition Geobacillus stearothermophilus
3.2.1.8 N-bromosuccinimide xylan protects from inactivation Geobacillus stearothermophilus
3.2.1.8 Ni2+ 10 mM, 49% inhibition Geobacillus stearothermophilus
3.2.1.8 Zn2+
-
 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information
-
 Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Geobacillus stearothermophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 41900
-
 gel filtration Geobacillus stearothermophilus
3.2.1.8 43000
-
 1 * 43000, SDS-PAGE Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Geobacillus stearothermophilus
-
-
-
3.2.1.8 Geobacillus stearothermophilus T-6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Geobacillus stearothermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 1,4-beta-D-xylan + H2O
-
 Geobacillus stearothermophilus additional information xylose + xylobiose ?
3.2.1.8 1,4-beta-D-xylan + H2O
-
 Geobacillus stearothermophilus T-6 additional information xylose + xylobiose ?
3.2.1.8 carboxymethylcellulose + H2O very low activity Geobacillus stearothermophilus ?
-
 ?
3.2.1.8 carboxymethylcellulose + H2O very low activity Geobacillus stearothermophilus T-6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 43000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 75
-
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 45 85 45°C: 28% of maximal activity, 85°C: 74% of maximal activity Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 65
-
 pH 7.0, 10 h, stable, pH 9.0, half-life is 6 h Geobacillus stearothermophilus
3.2.1.8 70
-
 pH 7.0, half-life is 14.5 h Geobacillus stearothermophilus
3.2.1.8 75
-
 pH 7.0, half-life is 20 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
-
 Geobacillus stearothermophilus
3.2.1.8 6.5 7
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 11 pH 5.0: about 45% of maximal activity, pH 8.0: about 55% of maximal activity, pH 10.0: about 60% of maximal activity, pH 11.0: about 45% of maximal activity Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 7
-
 65°C, stable for more than 10 h Geobacillus stearothermophilus
3.2.1.8 9
-
 half-life: 6 h Geobacillus stearothermophilus