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Literature summary extracted from

  • Ghosh, A.K.; Banerjee, P.C.; Sengupta, S.
    Purification and properties of xylan hydrolase from mushroom Termitomyces clypeatus (1980), Biochim. Biophys. Acta, 612, 143-152.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.8 15% loss of activity per freezing and thawing cycle Termitomyces clypeatus
3.2.1.8 stable towards lyophilization Termitomyces clypeatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+
-
 Termitomyces clypeatus
3.2.1.8 Ca2+ 20 mM, mild inhibition Termitomyces clypeatus
3.2.1.8 Cu2+ 10 mM, mild inhibition Termitomyces clypeatus
3.2.1.8 EDTA strong inhibition at 20 mM, weak inhibition at 2 mM Termitomyces clypeatus
3.2.1.8 Fe2+
-
 Termitomyces clypeatus
3.2.1.8 Hg2+
-
 Termitomyces clypeatus
3.2.1.8 iodoacetate strong inhibition at 20 mM, weak inhibition at 2 mM Termitomyces clypeatus
3.2.1.8 NaH2PO4 100 mM, 50% inhibition Termitomyces clypeatus
3.2.1.8 Zn2+ mild inhibition Termitomyces clypeatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information
-
 Termitomyces clypeatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 90000
-
 x * 90000, SDS-PAGE Termitomyces clypeatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Termitomyces clypeatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Termitomyces clypeatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 55.5
-
-
 Termitomyces clypeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 amylopectin + H2O
-
 Termitomyces clypeatus ?
-
 ?
3.2.1.8 arabinogalactan + H2O
-
 Termitomyces clypeatus ?
-
 ?
3.2.1.8 arabinoxylan + H2O
-
 Termitomyces clypeatus additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 90000, SDS-PAGE Termitomyces clypeatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 55
-
-
 Termitomyces clypeatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 35
-
 1 h, stable Termitomyces clypeatus
3.2.1.8 40
-
 4 h, 65% loss of activity Termitomyces clypeatus
3.2.1.8 60
-
 stable up to Termitomyces clypeatus
3.2.1.8 70
-
 1 h, complete loss of activity Termitomyces clypeatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 3 7 about 50% of maximal activity at pH 3.0 and at pH 7.0 Termitomyces clypeatus
3.2.1.8 5.5
-
-
 Termitomyces clypeatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 3 10 1 h, stable Termitomyces clypeatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.8 100
-
 NaH2PO4
-
 Termitomyces clypeatus