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Literature summary extracted from

  • Krishnapillai, A.M.; Taylor, K.D.A.; Morris, A.E.J.; Quantick, P.C.
    Characterisation of Norway lobster (Nephrops norvegicus) hyaluronidase and comparison with sheep and bovine testicular hyaluronidase (1999), Food Chem., 65, 515-521.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.35 bovine serum albumin enhanced activity of 22% at 0.26 mg/ml Nephrops norvegicus
3.2.1.35 EDTA up to 24% enhanced activity Nephrops norvegicus
3.2.1.35 NaCl 0.12 M required for optimum activity Nephrops norvegicus

General Stability

EC Number General Stability Organism
3.2.1.35 enzyme activity lost by freezing, addition of bovine serum albumin or 1% dimethylsulfoxide, v/v, prevents inactivation Nephrops norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.35 Ag+ 82% inhibition at 0.8 mM Nephrops norvegicus
3.2.1.35 chondroitin O-sulfate complete inhibition from 0.012-1.2 mg/ml Nephrops norvegicus
3.2.1.35 Cu2+ complete inhibition at 0.8 mM Nephrops norvegicus
3.2.1.35 cysteine complete inhibition from 0.012-1.2 mg/ml Nephrops norvegicus
3.2.1.35 Fe2+ complete inhibition at 0.8 mM Nephrops norvegicus
3.2.1.35 Fe3+ complete inhibition at 0.8 mM Nephrops norvegicus
3.2.1.35 heparin 58.4% inhibition at 1.2 mg/ml Nephrops norvegicus
3.2.1.35 Hg2+ complete inhibition at 0.8 mM Nephrops norvegicus
3.2.1.35 serum protein from human, 33.7% inhibition at 1.2 mg/ml Nephrops norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.35 additional information
-
 hyaluronoic acid value in mg/ml Nephrops norvegicus
3.2.1.35 additional information
-
 hyaluronoic acid 0.48 mg/ml Nephrops norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.35 Co2+ 18% enhanced activity at 0.8 mM Nephrops norvegicus
3.2.1.35 NO3- 18% enhanced activity at 0.8 mM Nephrops norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.35 320000
-
 gel filtration, native PAGE Nephrops norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.35 hyaluronic acid + H2O Nephrops norvegicus endo-mucopolysaccharidase hydrolysing acid mucopolysaccharides from connective tissue and chondroitin-sulfate in cartilage ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Nephrops norvegicus
-
 Norway lobster
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.35 partial Nephrops norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 hepatopancreas
-
 Nephrops norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 chitin + H2O 12 h incubation Nephrops norvegicus ?
-
 ?
3.2.1.35 hyaluronic acid + H2O endo-mucopolysaccharidase hydrolysing acid mucopolysaccharides from connective tissue and chondroitin-sulfate in cartilage Nephrops norvegicus ?
-
 ?
3.2.1.35 hyaluronic acid + H2O
-
 Nephrops norvegicus hyaluronic acid oligomers
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 45
-
 sharp decrease of activity above Nephrops norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 5.4
-
 in 50 mM sodium-acetate buffer, optimum pH depends on buffer used Nephrops norvegicus