EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.33 | 2-(N-morpholino)ethanesulfonic acid | - |
Oryctolagus cuniculus | |
3.2.1.33 | Amine | protonated, hydroxylalkyl-substituted, noncompetitive, mechanism of inhibition | Oryctolagus cuniculus | |
3.2.1.33 | cationic buffer | - |
Oryctolagus cuniculus | |
3.2.1.33 | choline | very poor inhibitor | Oryctolagus cuniculus | |
3.2.1.33 | diethanolamine | - |
Oryctolagus cuniculus | |
3.2.1.33 | ethanolamine | - |
Oryctolagus cuniculus | |
3.2.1.33 | ethylamine | - |
Oryctolagus cuniculus | |
3.2.1.33 | glycylglycine | no inhibition at 0.01 or 0.05 M | Oryctolagus cuniculus | |
3.2.1.33 | hydroxylamine | - |
Oryctolagus cuniculus | |
3.2.1.33 | imidazole | - |
Oryctolagus cuniculus | |
3.2.1.33 | additional information | inhibition mechanism | Oryctolagus cuniculus | |
3.2.1.33 | N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid | slight inhibition | Oryctolagus cuniculus | |
3.2.1.33 | N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid | slight inhibition | Oryctolagus cuniculus | |
3.2.1.33 | NH4+ | slight inhibition | Oryctolagus cuniculus | |
3.2.1.33 | taurine | slight inhibition | Oryctolagus cuniculus | |
3.2.1.33 | triethylamine | very poor inhibitor | Oryctolagus cuniculus | |
3.2.1.33 | Tris | - |
Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.33 | additional information | - |
additional information | - |
Oryctolagus cuniculus | |
3.2.1.33 | 0.063 | - |
glycogen phosphorylase limit dextrin | - |
Oryctolagus cuniculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.33 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.33 | glycogen debranching enzyme | Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.33 | muscle | - |
Oryctolagus cuniculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 8.3 | - |
- |
Oryctolagus cuniculus |
3.2.1.33 | 9.5 | - |
- |
Oryctolagus cuniculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.33 | 63-alpha-glucosyl maltotetraose + H2O | branched pentasaccharide "fast B5" | Oryctolagus cuniculus | maltotetraose + D-glucose | - |
r | |
3.2.1.33 | alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Oryctolagus cuniculus | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
3.2.1.33 | amylopectin + H2O | very poor substrate | Oryctolagus cuniculus | amylopectin + D-glucose | - |
r | |
3.2.1.33 | glycogen + H2O | rabbit liver glycogen: slowly | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
3.2.1.33 | glycogen phosphorylase limit dextrin + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
3.2.1.33 | glycogen phosphorylase-limit dextrin + H2O | - |
Oryctolagus cuniculus | limit dextrin + D-glucose | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.33 | glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Oryctolagus cuniculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 6 | - |
phosphate-citrate buffer | Oryctolagus cuniculus |
3.2.1.33 | 6.6 | - |
anionic buffer, substrate: glycogen phosphorylase limit dextrin | Oryctolagus cuniculus |
3.2.1.33 | 6.6 | - |
imidazole buffer | Oryctolagus cuniculus |
3.2.1.33 | 7.2 | - |
cationic buffer, substrate: glycogen phosphorylase limit dextrin | Oryctolagus cuniculus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.33 | 5 | 8 | - |
Oryctolagus cuniculus |