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Literature summary extracted from

  • Huber, R.E.; Roth, N.J.; Bahl, H.
    Quarternary structure, Mg2+ interactions, and some kinetic properties of the beta-galactosidase from Thermoanaerobacterium thermosulfurigenes EM1 (1996), J. Protein Chem., 15, 621-629.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 2-deoxy-D-galactose
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.23 D-arabinose
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.23 D-galactono-1,4-lactone
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.23 D-galactose
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.23 D-glucose
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.23 D-Lyxose
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.23 glycerol
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.23 isopropyl thiogalactoside
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.23 lactose
-
 Thermoanaerobacterium thermosulfurigenes
3.2.1.23 ribose
-
 Thermoanaerobacterium thermosulfurigenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.37
-
 p-nitrophenyl-beta-D-galactoside at 50°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
3.2.1.23 0.39
-
 p-nitrophenyl-beta-D-galactoside at 25°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
3.2.1.23 0.58
-
 o-nitrophenyl-beta-D-galactoside at 50°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
3.2.1.23 0.64
-
 o-nitrophenyl-beta-D-galactoside at 25°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ no effect Thermoanaerobacterium thermosulfurigenes
3.2.1.23 Mg2+ contains 0.19 Mg2+ per monomer Thermoanaerobacterium thermosulfurigenes
3.2.1.23 Na+ no effect Thermoanaerobacterium thermosulfurigenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 175000 178000 gel filtration, non-denaturing PAGE Thermoanaerobacterium thermosulfurigenes

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Thermoanaerobacterium thermosulfurigenes
-
-
-
3.2.1.23 Thermoanaerobacterium thermosulfurigenes EM1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacterium thermosulfurigenes o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacterium thermosulfurigenes EM1 o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacterium thermosulfurigenes p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacterium thermosulfurigenes EM1 p-nitrophenol + beta-D-galactose
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 42.4
-
 p-nitrophenyl-beta-D-galactoside at 25°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
3.2.1.23 54.5
-
 o-nitrophenyl-beta-D-galactoside at 25°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
3.2.1.23 264
-
 o-nitrophenyl-beta-D-galactoside at 50°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
3.2.1.23 312
-
 p-nitrophenyl-beta-D-galactoside at 50°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.5
-
-
 Thermoanaerobacterium thermosulfurigenes