EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | 2-mercaptoethanol | - |
Thermus sp. | |
3.2.1.23 | Cys | - |
Thermus sp. | |
3.2.1.23 | dithiothreitol | - |
Thermus sp. | |
3.2.1.23 | EDTA | activates | Thermus sp. | |
3.2.1.23 | phenylmethyl sulfonyl fluoride | - |
Thermus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | cellobiose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
3.2.1.23 | Cu+ | - |
Thermus sp. | |
3.2.1.23 | Cu2+ | - |
Thermus sp. | |
3.2.1.23 | D-galactose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
3.2.1.23 | D-glucose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
3.2.1.23 | Fe2+ | - |
Thermus sp. | |
3.2.1.23 | Fe3+ | weak | Thermus sp. | |
3.2.1.23 | iodoacetate | - |
Thermus sp. | |
3.2.1.23 | lactose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
3.2.1.23 | Mn2+ | weak | Thermus sp. | |
3.2.1.23 | Ni2+ | weak | Thermus sp. | |
3.2.1.23 | PCMB | - |
Thermus sp. | |
3.2.1.23 | xylose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
3.2.1.23 | Zn2+ | - |
Thermus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 5.5 | - |
o-nitrophenyl-beta-D-galactoside | soluble and immobilized enzyme | Thermus sp. | |
3.2.1.23 | 43 | - |
lactose | soluble and immobilized enzyme | Thermus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | additional information | Thermus sp. | inducible enzyme | ? | - |
? | |
3.2.1.23 | additional information | Thermus sp. 4-1A | inducible enzyme | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.23 | Thermus sp. | - |
- |
- |
3.2.1.23 | Thermus sp. 4-1A | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.23 | partial | Thermus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 62.83 | - |
- |
Thermus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | additional information | inducible enzyme | Thermus sp. | ? | - |
? | |
3.2.1.23 | additional information | inducible enzyme | Thermus sp. 4-1A | ? | - |
? | |
3.2.1.23 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus sp. | o-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus sp. 4-1A | o-nitrophenol + beta-D-galactose | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 90 | - |
at pH 8, stable up to | Thermus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 6 | - |
soluble and immobilized enzyme | Thermus sp. |