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Literature summary extracted from

  • Cowan, D.A.; Daniel, R.M.; Martin, A.M.; Morgan, H.W.
    Some properties of a beta-galactosidase from an extremely thermophilic bacterium (1984), Biotechnol. Bioeng., 26, 1141-1145.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 2-mercaptoethanol
-
Thermus sp.
3.2.1.23 Cys
-
Thermus sp.
3.2.1.23 dithiothreitol
-
Thermus sp.
3.2.1.23 EDTA activates Thermus sp.
3.2.1.23 phenylmethyl sulfonyl fluoride
-
Thermus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 cellobiose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
3.2.1.23 Cu+
-
Thermus sp.
3.2.1.23 Cu2+
-
Thermus sp.
3.2.1.23 D-galactose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
3.2.1.23 D-glucose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
3.2.1.23 Fe2+
-
Thermus sp.
3.2.1.23 Fe3+ weak Thermus sp.
3.2.1.23 iodoacetate
-
Thermus sp.
3.2.1.23 lactose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
3.2.1.23 Mn2+ weak Thermus sp.
3.2.1.23 Ni2+ weak Thermus sp.
3.2.1.23 PCMB
-
Thermus sp.
3.2.1.23 xylose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
3.2.1.23 Zn2+
-
Thermus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 5.5
-
o-nitrophenyl-beta-D-galactoside soluble and immobilized enzyme Thermus sp.
3.2.1.23 43
-
lactose soluble and immobilized enzyme Thermus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.23 additional information Thermus sp. inducible enzyme ?
-
?
3.2.1.23 additional information Thermus sp. 4-1A inducible enzyme ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Thermus sp.
-
-
-
3.2.1.23 Thermus sp. 4-1A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 partial Thermus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 62.83
-
-
Thermus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 additional information inducible enzyme Thermus sp. ?
-
?
3.2.1.23 additional information inducible enzyme Thermus sp. 4-1A ?
-
?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
Thermus sp. o-nitrophenol + beta-D-galactose
-
?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
Thermus sp. 4-1A o-nitrophenol + beta-D-galactose
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 90
-
at pH 8, stable up to Thermus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6
-
soluble and immobilized enzyme Thermus sp.