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Literature summary extracted from

  • Konno, H.; Yamasaki, Y.; Katoh, K.
    Purification and characterization of beta-galactosidase from cell suspension cultures of Marchantia polymorpha (1986), Plant Sci., 44, 97-104.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ag2+
-
 Marchantia polymorpha
3.2.1.23 Cu2+
-
 Marchantia polymorpha
3.2.1.23 D-galactono-1,4-lactone
-
 Marchantia polymorpha
3.2.1.23 D-galactose
-
 Marchantia polymorpha
3.2.1.23 D-glucono-delta-lactone
-
 Marchantia polymorpha
3.2.1.23 Hg2+
-
 Marchantia polymorpha

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.625
-
 p-nitrophenyl beta-D-galactopyranoside
-
 Marchantia polymorpha

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 62000
-
 1 * 62000, SDS-PAGE Marchantia polymorpha
3.2.1.23 67000
-
 gel filtration Marchantia polymorpha

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Marchantia polymorpha
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Marchantia polymorpha

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 cell culture
-
 Marchantia polymorpha
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 additional information
-
-
 Marchantia polymorpha

Storage Stability

EC Number Storage Stability Organism
3.2.1.23 -20°C, stable for at least 2 months Marchantia polymorpha

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 galactan + H2O
-
 Marchantia polymorpha galactose + small amounts of glucose and arabinose ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + D-glucose transgalactosylation Marchantia polymorpha lactose + p-nitrophenol
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Marchantia polymorpha p-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 monomer 1 * 62000, SDS-PAGE Marchantia polymorpha

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
-
 Marchantia polymorpha

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 50
-
 pH 4.0, 20 min, 50% loss of activity Marchantia polymorpha
3.2.1.23 60
-
 pH 4.0, 20 min, complete loss of activity Marchantia polymorpha

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 2.6
-
 glycine/HCl buffer Marchantia polymorpha
3.2.1.23 3.4
-
 McIlvaine buffer Marchantia polymorpha

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 2.2 4.6 about 50% of maximal activity at pH 2.2 and at pH 4.6 Marchantia polymorpha

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 6 7 30°C, 16 h, stable Marchantia polymorpha