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Literature summary extracted from

  • Sekimata, M.; Ogura, K.; Tsumuraya, Y.; Hashimoto, Y.; Yamamoto, S.
    A beta-galactosidase from radish (Raphanus sativus L.) seeds (1989), Plant Physiol., 90, 567-574.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 Triton X-100 0.01%, enhances activity Raphanus sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ag+ 1 mM, almost complete inhibition Raphanus sativus
3.2.1.23 Cu2+ 1 mM, almost complete inhibition Raphanus sativus
3.2.1.23 D-arabinose 10 mM, 15% inhibition Raphanus sativus
3.2.1.23 D-fucose 10 mM, 20% inhibition Raphanus sativus
3.2.1.23 D-galactal potent, competitive Raphanus sativus
3.2.1.23 D-galactono-1,4-lactone potent, competitive Raphanus sativus
3.2.1.23 D-galactose 10 mM D-galactose, 60% inhibition Raphanus sativus
3.2.1.23 Fe3+ 1 mM, almost complete inhibition Raphanus sativus
3.2.1.23 Hg2+ 1 mM, almost complete inhibition Raphanus sativus
3.2.1.23 iodoacetate 1 mM, 52% inhibition Raphanus sativus
3.2.1.23 L-arabinose 10 mM, 18% inhibition Raphanus sativus
3.2.1.23 L-fucose 10 mM, 30% inhibition Raphanus sativus
3.2.1.23 Mn2+ 1 mM, almost complete inhibition Raphanus sativus
3.2.1.23 PCMB
-
 Raphanus sativus
3.2.1.23 SDS 0.01%, complete inactivation Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.46
-
 p-nitrophenyl-beta-D-galactoside
-
 Raphanus sativus
3.2.1.23 1.19
-
 o-nitrophenyl-beta-D-galactoside
-
 Raphanus sativus
3.2.1.23 1.27
-
 4-methylumbelliferyl-beta-D-galactoside
-
 Raphanus sativus
3.2.1.23 4.87
-
 beta-1,3-galactotriose
-
 Raphanus sativus
3.2.1.23 7.79
-
 beta-1,3-galactobiose
-
 Raphanus sativus
3.2.1.23 8.55
-
 lactose
-
 Raphanus sativus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Raphanus sativus
-
 L. var. hortensis cv. Aokubi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 radicle epidermal cells, intercellular space of cortical cells, endodermis surrounding a vascular bundle Raphanus sativus
-
3.2.1.23 seed
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 5.62
-
-
 Raphanus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 4-methylumbelliferyl-beta-D-galactoside + H2O
-
 Raphanus sativus 4-methylumbelliferone + beta-D-galactose
-
 ?
3.2.1.23 beta-1,3-galactobiose + H2O
-
 Raphanus sativus beta-D-galactose
-
 ?
3.2.1.23 beta-1,3-galactotriose + H2O
-
 Raphanus sativus ?
-
 ?
3.2.1.23 beta-1,6-galactobiose + H2O
-
 Raphanus sativus beta-D-galactose
-
 ?
3.2.1.23 beta-1,6-galactotriose + H2O
-
 Raphanus sativus ?
-
 ?
3.2.1.23 beta-D-Galp-(1->3)-beta-D-Galp-(1->3)-D-galactitol + H2O
-
 Raphanus sativus beta-D-Galp-(1->3)-D-galactitol + D-galactopyranose
-
 ?
3.2.1.23 beta-D-Galp-(1->6)-beta-D-Galp-(1->6)-D-galactitol + H2O
-
 Raphanus sativus beta-D-Galp-(1->6)-D-galactitol + D-galactopyranose
-
 ?
3.2.1.23 lactose + H2O
-
 Raphanus sativus D-glucose + D-galactose
-
 ?
3.2.1.23 methyl beta-D-galactohexaoside + H2O beta-1,6-linked Raphanus sativus ?
-
 ?
3.2.1.23 methyl beta-D-galactopentaoside + H2O beta-1,3-linked or beta-1,6-linked Raphanus sativus ?
-
 ?
3.2.1.23 methyl beta-D-galactotetraoside + H2O beta-1,3-linked Raphanus sativus ?
-
 ?
3.2.1.23 methyl O-beta-galactoside + H2O
-
 Raphanus sativus methanol + beta-D-galactose
-
 ?
3.2.1.23 additional information specifically splits off beta-1,3-linked and beta-1,6-linked D-galactosyl residues from the nonreducing ends, their rates of hydrolysis increases with increasing chain length Raphanus sativus ?
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Raphanus sativus o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Raphanus sativus p-nitrophenol + beta-D-galactose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 40
-
 pH 4.0, 10 min, stable up to Raphanus sativus
3.2.1.23 55
-
 pH 4.0, 10 min, complete inactivation Raphanus sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4
-
 hydrolysis of p-nitrophenyl-beta-D-galactoside or beta-1,3-galactobiose Raphanus sativus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 3.5 8.5 4°C, 24 h, stable Raphanus sativus