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Literature summary extracted from

  • Lazo, P.S.; Ochoa, A.G.; Gascon, S.
    alpha-Galactosidase (melibiase) from Saccharomyces carlsbergensis: structural and kinetic properties (1978), Arch. Biochem. Biophys., 191, 316-324.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 D-fucose
-
 Saccharomyces pastorianus
3.2.1.22 D-galactose
-
 Saccharomyces pastorianus
3.2.1.22 D-glucose
-
 Saccharomyces pastorianus
3.2.1.22 L-arabinose
-
 Saccharomyces pastorianus
3.2.1.22 melibiose
-
 Saccharomyces pastorianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 6
-
 melibiose
-
 Saccharomyces pastorianus
3.2.1.22 6
-
 p-nitrophenyl alpha-D-galactoside
-
 Saccharomyces pastorianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 300000
-
 gel filtration Saccharomyces pastorianus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Saccharomyces pastorianus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.22 glycoprotein
-
 Saccharomyces pastorianus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Saccharomyces pastorianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O
-
 Saccharomyces pastorianus D-galactose + D-glucose
-
 ?
3.2.1.22 p-nitrophenyl-alpha-D-galactoside + H2O
-
 Saccharomyces pastorianus p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Saccharomyces pastorianus sucrose + D-galactose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4 5
-
 Saccharomyces pastorianus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 2 9
-
 Saccharomyces pastorianus