BRENDA - Enzyme Database

Purification, characterization and modulation of a microsomal carboxylesterase in rat liver for the hydrolysis of acyl-CoA

Mukherjee, J.J.; Jay, F.T.; Choy, P.C.; Biochem. J. 295, 81-86 (1993)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.1
lysophosphatidylcholine
activate
Rattus norvegicus
3.1.1.1
phosphatidylcholine
activates
Rattus norvegicus
3.1.1.1
phosphatidylethanolamine
activates
Rattus norvegicus
3.1.2.2
lysophosphatidylcholine
-
Rattus norvegicus
3.1.2.2
lysophosphatidylethanolamine
-
Rattus norvegicus
3.1.2.2
phosphatidylcholine
-
Rattus norvegicus
3.1.2.2
phosphatidylethanolamine
-
Rattus norvegicus
3.1.2.20
lysophosphatidylcholine
-
Rattus norvegicus
3.1.2.20
lysophosphatidylethanolamine
-
Rattus norvegicus
3.1.2.20
phosphatidylcholine
-
Rattus norvegicus
3.1.2.20
phosphatidylethanolamine
-
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.1
lysophosphatidic acid
-
Rattus norvegicus
3.1.2.2
cardiolipin
-
Rattus norvegicus
3.1.2.2
lysophosphatidic acid
-
Rattus norvegicus
3.1.2.20
cardiolipin
-
Rattus norvegicus
3.1.2.20
lysophosphatidic acid
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.1
0.017
-
palmitoyl-CoA
-
Rattus norvegicus
3.1.2.2
0.017
-
hexadecanoyl-CoA
-
Rattus norvegicus
3.1.2.20
0.017
-
hexadecanoyl-CoA
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.1
microsome
-
Rattus norvegicus
-
-
3.1.2.2
microsome
-
Rattus norvegicus
-
-
3.1.2.20
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.1
56000
-
1 * 56000, SDS-PAGE
Rattus norvegicus
3.1.1.1
64000
-
gel filtration
Rattus norvegicus
3.1.2.2
56000
-
x * 56000, SDS-PAGE
Rattus norvegicus
3.1.2.2
64000
-
gel filtration
Rattus norvegicus
3.1.2.20
56000
-
x * 56000, SDS-PAGE
Rattus norvegicus
3.1.2.20
64000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.2
palmitoyl-CoA + H2O
Rattus norvegicus
-
CoA + palmitate
-
-
?
3.1.2.20
palmitoyl-CoA + H2O
Rattus norvegicus
-
CoA + palmitate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.1
Rattus norvegicus
-
-
-
3.1.2.2
Rattus norvegicus
-
-
-
3.1.2.20
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.1
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.1
liver
-
Rattus norvegicus
-
3.1.2.2
liver
-
Rattus norvegicus
-
3.1.2.20
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.1
3.1
-
hydrolysis of palmitoyl-CoA
Rattus norvegicus
3.1.1.1
66.7
-
hydrolysis of 4-nitrophenyl acetate
Rattus norvegicus
3.1.2.2
3.1
-
-
Rattus norvegicus
3.1.2.2
66.7
-
-
Rattus norvegicus
3.1.2.20
3.1
-
-
Rattus norvegicus
3.1.2.20
66.7
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
3.1.2.2
-70°C in the presence of 20% glycerol over 1 month
Rattus norvegicus
3.1.2.20
-70°C in the presence of 20% glycerol over 1 month
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.1
4-nitrophenyl acetate + H2O
-
171023
Rattus norvegicus
4-nitrophenol + acetate
-
-
-
?
3.1.1.1
oleoyl-CoA + H2O
-
171023
Rattus norvegicus
oleic acid + CoA
-
-
-
?
3.1.1.1
palmitoyl-CoA + H2O
-
171023
Rattus norvegicus
palmitic acid + CoA
-
-
-
?
3.1.1.1
stearoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + stearate
-
-
-
?
3.1.2.2
4-nitrophenyl acetate + H2O
-
171023
Rattus norvegicus
4-nitrophenol + acetate
-
-
-
?
3.1.2.2
acyl-CoA + H2O
long-chain fatty-acyl-CoA
171023
Rattus norvegicus
CoA + a carboxylate
-
-
-
?
3.1.2.2
hexadecanoyl-CoA + H2O
best substrate
171023
Rattus norvegicus
CoA + hexadecanoate
-
-
-
?
3.1.2.2
hexadecanoyl-CoA + H2O
i.e. palmitoyl-CoA
171023
Rattus norvegicus
CoA + hexadecanoate
-
-
-
?
3.1.2.2
octadecanoyl-CoA + H2O
i.e. stearoyl-CoA
171023
Rattus norvegicus
CoA + octadecanoate
-
-
-
?
3.1.2.2
oleoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + oleate
-
-
-
?
3.1.2.2
palmitoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + palmitate
-
-
-
?
3.1.2.20
4-nitrophenyl acetate + H2O
-
171023
Rattus norvegicus
4-nitrophenol + acetate
-
-
-
?
3.1.2.20
acyl-CoA + H2O
long-chain fatty-acyl-CoA
171023
Rattus norvegicus
CoA + a carboxylate
-
-
-
?
3.1.2.20
hexadecanoyl-CoA + H2O
best substrate
171023
Rattus norvegicus
CoA + hexadecanoate
-
-
-
?
3.1.2.20
hexadecanoyl-CoA + H2O
i.e. palmitoyl-CoA
171023
Rattus norvegicus
CoA + hexadecanoate
-
-
-
?
3.1.2.20
octadecanoyl-CoA + H2O
i.e. stearoyl-CoA
171023
Rattus norvegicus
CoA + octadecanoate
-
-
-
?
3.1.2.20
oleoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + oleate
-
-
-
?
3.1.2.20
palmitoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + palmitate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.1
monomer
1 * 56000, SDS-PAGE
Rattus norvegicus
3.1.2.2
?
x * 56000, SDS-PAGE
Rattus norvegicus
3.1.2.20
?
x * 56000, SDS-PAGE
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.1
lysophosphatidylcholine
activate
Rattus norvegicus
3.1.1.1
phosphatidylcholine
activates
Rattus norvegicus
3.1.1.1
phosphatidylethanolamine
activates
Rattus norvegicus
3.1.2.2
lysophosphatidylcholine
-
Rattus norvegicus
3.1.2.2
lysophosphatidylethanolamine
-
Rattus norvegicus
3.1.2.2
phosphatidylcholine
-
Rattus norvegicus
3.1.2.2
phosphatidylethanolamine
-
Rattus norvegicus
3.1.2.20
lysophosphatidylcholine
-
Rattus norvegicus
3.1.2.20
lysophosphatidylethanolamine
-
Rattus norvegicus
3.1.2.20
phosphatidylcholine
-
Rattus norvegicus
3.1.2.20
phosphatidylethanolamine
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.1
lysophosphatidic acid
-
Rattus norvegicus
3.1.2.2
cardiolipin
-
Rattus norvegicus
3.1.2.2
lysophosphatidic acid
-
Rattus norvegicus
3.1.2.20
cardiolipin
-
Rattus norvegicus
3.1.2.20
lysophosphatidic acid
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.1
0.017
-
palmitoyl-CoA
-
Rattus norvegicus
3.1.2.2
0.017
-
hexadecanoyl-CoA
-
Rattus norvegicus
3.1.2.20
0.017
-
hexadecanoyl-CoA
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.1
microsome
-
Rattus norvegicus
-
-
3.1.2.2
microsome
-
Rattus norvegicus
-
-
3.1.2.20
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.1
56000
-
1 * 56000, SDS-PAGE
Rattus norvegicus
3.1.1.1
64000
-
gel filtration
Rattus norvegicus
3.1.2.2
56000
-
x * 56000, SDS-PAGE
Rattus norvegicus
3.1.2.2
64000
-
gel filtration
Rattus norvegicus
3.1.2.20
56000
-
x * 56000, SDS-PAGE
Rattus norvegicus
3.1.2.20
64000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.2.2
palmitoyl-CoA + H2O
Rattus norvegicus
-
CoA + palmitate
-
-
?
3.1.2.20
palmitoyl-CoA + H2O
Rattus norvegicus
-
CoA + palmitate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.1
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.1
liver
-
Rattus norvegicus
-
3.1.2.2
liver
-
Rattus norvegicus
-
3.1.2.20
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.1
3.1
-
hydrolysis of palmitoyl-CoA
Rattus norvegicus
3.1.1.1
66.7
-
hydrolysis of 4-nitrophenyl acetate
Rattus norvegicus
3.1.2.2
3.1
-
-
Rattus norvegicus
3.1.2.2
66.7
-
-
Rattus norvegicus
3.1.2.20
3.1
-
-
Rattus norvegicus
3.1.2.20
66.7
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.2.2
-70°C in the presence of 20% glycerol over 1 month
Rattus norvegicus
3.1.2.20
-70°C in the presence of 20% glycerol over 1 month
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.1
4-nitrophenyl acetate + H2O
-
171023
Rattus norvegicus
4-nitrophenol + acetate
-
-
-
?
3.1.1.1
oleoyl-CoA + H2O
-
171023
Rattus norvegicus
oleic acid + CoA
-
-
-
?
3.1.1.1
palmitoyl-CoA + H2O
-
171023
Rattus norvegicus
palmitic acid + CoA
-
-
-
?
3.1.1.1
stearoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + stearate
-
-
-
?
3.1.2.2
4-nitrophenyl acetate + H2O
-
171023
Rattus norvegicus
4-nitrophenol + acetate
-
-
-
?
3.1.2.2
acyl-CoA + H2O
long-chain fatty-acyl-CoA
171023
Rattus norvegicus
CoA + a carboxylate
-
-
-
?
3.1.2.2
hexadecanoyl-CoA + H2O
best substrate
171023
Rattus norvegicus
CoA + hexadecanoate
-
-
-
?
3.1.2.2
hexadecanoyl-CoA + H2O
i.e. palmitoyl-CoA
171023
Rattus norvegicus
CoA + hexadecanoate
-
-
-
?
3.1.2.2
octadecanoyl-CoA + H2O
i.e. stearoyl-CoA
171023
Rattus norvegicus
CoA + octadecanoate
-
-
-
?
3.1.2.2
oleoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + oleate
-
-
-
?
3.1.2.2
palmitoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + palmitate
-
-
-
?
3.1.2.20
4-nitrophenyl acetate + H2O
-
171023
Rattus norvegicus
4-nitrophenol + acetate
-
-
-
?
3.1.2.20
acyl-CoA + H2O
long-chain fatty-acyl-CoA
171023
Rattus norvegicus
CoA + a carboxylate
-
-
-
?
3.1.2.20
hexadecanoyl-CoA + H2O
best substrate
171023
Rattus norvegicus
CoA + hexadecanoate
-
-
-
?
3.1.2.20
hexadecanoyl-CoA + H2O
i.e. palmitoyl-CoA
171023
Rattus norvegicus
CoA + hexadecanoate
-
-
-
?
3.1.2.20
octadecanoyl-CoA + H2O
i.e. stearoyl-CoA
171023
Rattus norvegicus
CoA + octadecanoate
-
-
-
?
3.1.2.20
oleoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + oleate
-
-
-
?
3.1.2.20
palmitoyl-CoA + H2O
-
171023
Rattus norvegicus
CoA + palmitate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.1
monomer
1 * 56000, SDS-PAGE
Rattus norvegicus
3.1.2.2
?
x * 56000, SDS-PAGE
Rattus norvegicus
3.1.2.20
?
x * 56000, SDS-PAGE
Rattus norvegicus