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Literature summary extracted from

  • Manco, G.; di Gennaro, S.; de Rosa, M.; Rossi, M.
    Purification and characterization of a thermostable carboxylesterase from the thermoacidophilic eubacterium Bacillus acidocaldarius (1994), Eur. J. Biochem., 221, 965-972.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 diethyldicarbonate
-
 Alicyclobacillus acidocaldarius
3.1.1.1 DTT 1 mM 12% inhibition Alicyclobacillus acidocaldarius
3.1.1.1 EDTA 10 mM, 20% inhibition Alicyclobacillus acidocaldarius
3.1.1.1 paraoxon
-
 Alicyclobacillus acidocaldarius
3.1.1.1 physostigmine
-
 Alicyclobacillus acidocaldarius
3.1.1.1 PMSF
-
 Alicyclobacillus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.0126
-
 4-nitrophenyl hexanoate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0165
-
 4-nitrophenyl octanoate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0192
-
 4-nitrophenyl hexanoate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0254
-
 4-nitrophenyl octanoate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0335
-
 4-nitrophenyl octanoate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0375
-
 4-nitrophenyl hexanoate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0566
-
 4-nitrophenyl pentanoate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0705
-
 4-nitrophenyl butyrate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0756
-
 4-nitrophenyl butyrate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0766
-
 4-nitrophenyl pentanoate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 0.08
-
 4-nitrophenyl pentanoate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0828
-
 4-nitrophenyl propionate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0853
-
 4-nitrophenyl acetate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 0.0983
-
 4-nitrophenyl butyrate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 0.125
-
 4-nitrophenyl acetate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 0.137
-
 4-nitrophenyl propionate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 0.183
-
 4-nitrophenyl acetate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 0.196
-
 4-nitrophenyl propionate 37°C Alicyclobacillus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 36500
-
 1 * 36500, SDS-PAGE Alicyclobacillus acidocaldarius
3.1.1.1 37500
-
 gel filtration Alicyclobacillus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Alicyclobacillus acidocaldarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Alicyclobacillus acidocaldarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 additional information
-
-
 Alicyclobacillus acidocaldarius

Storage Stability

EC Number Storage Stability Organism
3.1.1.1 -20°C, 20 mM Tris/HCl, pH 8, 200 mM NaCl, 18 months, stable Alicyclobacillus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Alicyclobacillus acidocaldarius 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butanoate + H2O
-
 Alicyclobacillus acidocaldarius 4-nitrophenol + butanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Alicyclobacillus acidocaldarius 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl pentanoate + H2O
-
 Alicyclobacillus acidocaldarius 4-nitrophenol + pentanoate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O
-
 Alicyclobacillus acidocaldarius 4-nitrophenol + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 36500, SDS-PAGE Alicyclobacillus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 70
-
-
 Alicyclobacillus acidocaldarius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 143
-
 4-nitrophenyl acetate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 145
-
 4-nitrophenyl acetate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 213
-
 4-nitrophenyl acetate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 692
-
 4-nitrophenyl octanoate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 830
-
 4-nitrophenyl propionate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 881
-
 4-nitrophenyl octanoate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 904
-
 4-nitrophenyl butyrate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 908
-
 4-nitrophenyl hexanoate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 1000
-
 4-nitrophenyl propionate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 1070
-
 4-nitrophenyl pentanoate 37°C Alicyclobacillus acidocaldarius
3.1.1.1 1150
-
 4-nitrophenyl butyrate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 1170
-
 4-nitrophenyl hexanoate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 1230
-
 4-nitrophenyl propionate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 1230
-
 4-nitrophenyl octanoate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 1400
-
 4-nitrophenyl pentanoate 45°C Alicyclobacillus acidocaldarius
3.1.1.1 1670
-
 4-nitrophenyl butyrate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 1710
-
 4-nitrophenyl hexanoate 60°C Alicyclobacillus acidocaldarius
3.1.1.1 2050
-
 4-nitrophenyl pentanoate 37°C Alicyclobacillus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7 8.5
-
 Alicyclobacillus acidocaldarius
3.1.1.1 8
-
-
 Alicyclobacillus acidocaldarius