BRENDA - Enzyme Database

Catalytic properties of an unspecific carboxylesterase (E1) from rat-liver microsomes

Arndt, R.; Krisch, K.; Eur. J. Biochem. 36, 129-134 (1973)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.1
0.0184
-
ethyl iodoacetate
-
Rattus norvegicus
3.1.1.1
0.0313
-
ethyl bromoacetate
-
Rattus norvegicus
3.1.1.1
0.083
-
ethyl chloroacetate
-
Rattus norvegicus
3.1.1.1
0.11
-
n-butyl n-butyrate
-
Rattus norvegicus
3.1.1.1
0.125
-
4-nitrophenyl acetate
-
Rattus norvegicus
3.1.1.1
0.132
-
procaine
-
Rattus norvegicus
3.1.1.1
0.25
-
hostacaine
-
Rattus norvegicus
3.1.1.1
0.263
-
4-nitrophenyl butyrate
-
Rattus norvegicus
3.1.1.1
0.333
-
ethyl fluoroacetate
-
Rattus norvegicus
3.1.1.1
1.22
-
Tyr ethyl ester
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.1
microsome
-
Rattus norvegicus
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.1
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.1
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.1
4-nitrophenyl acetate + H2O
-
171016
Rattus norvegicus
4-nitrophenol + acetate
-
-
-
?
3.1.1.1
4-nitrophenyl butyrate + H2O
-
171016
Rattus norvegicus
4-nitrophenol + butyrate
-
-
-
?
3.1.1.1
ethyl bromoacetate + H2O
-
171016
Rattus norvegicus
ethanol + bromoacetate
-
-
-
?
3.1.1.1
ethyl chloroacetate + H2O
-
171016
Rattus norvegicus
ethanol + chloroacetate
-
-
-
?
3.1.1.1
ethyl fluoroacetate + H2O
-
171016
Rattus norvegicus
ethanol + fluoroacetate
-
-
-
?
3.1.1.1
ethyl iodoacetate + H2O
-
171016
Rattus norvegicus
ethanol + iodoacetate
-
-
-
?
3.1.1.1
L-Tyr ethyl ester + H2O
-
171016
Rattus norvegicus
L-Tyr + ethanol
-
-
-
?
3.1.1.1
additional information
-
171016
Rattus norvegicus
?
-
-
-
-
3.1.1.1
n-butyl n-butyrate + H2O
-
171016
Rattus norvegicus
n-butanol + n-butyrate
-
-
-
?
3.1.1.1
procaine + H2O
-
171016
Rattus norvegicus
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.1
5.5
10
hydrolysis of methyl butyrate, at optimal methyl butyrate concentrations, 0.01 M
Rattus norvegicus
3.1.1.1
7.5
-
hydrolysis of methyl hutyrate, at high substrate concentrations, 0.5 M
Rattus norvegicus
3.1.1.1
8
8.5
hydrolysis of p-nitrophenyl acetate
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.1
0.0184
-
ethyl iodoacetate
-
Rattus norvegicus
3.1.1.1
0.0313
-
ethyl bromoacetate
-
Rattus norvegicus
3.1.1.1
0.083
-
ethyl chloroacetate
-
Rattus norvegicus
3.1.1.1
0.11
-
n-butyl n-butyrate
-
Rattus norvegicus
3.1.1.1
0.125
-
4-nitrophenyl acetate
-
Rattus norvegicus
3.1.1.1
0.132
-
procaine
-
Rattus norvegicus
3.1.1.1
0.25
-
hostacaine
-
Rattus norvegicus
3.1.1.1
0.263
-
4-nitrophenyl butyrate
-
Rattus norvegicus
3.1.1.1
0.333
-
ethyl fluoroacetate
-
Rattus norvegicus
3.1.1.1
1.22
-
Tyr ethyl ester
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.1
microsome
-
Rattus norvegicus
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.1
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.1
4-nitrophenyl acetate + H2O
-
171016
Rattus norvegicus
4-nitrophenol + acetate
-
-
-
?
3.1.1.1
4-nitrophenyl butyrate + H2O
-
171016
Rattus norvegicus
4-nitrophenol + butyrate
-
-
-
?
3.1.1.1
ethyl bromoacetate + H2O
-
171016
Rattus norvegicus
ethanol + bromoacetate
-
-
-
?
3.1.1.1
ethyl chloroacetate + H2O
-
171016
Rattus norvegicus
ethanol + chloroacetate
-
-
-
?
3.1.1.1
ethyl fluoroacetate + H2O
-
171016
Rattus norvegicus
ethanol + fluoroacetate
-
-
-
?
3.1.1.1
ethyl iodoacetate + H2O
-
171016
Rattus norvegicus
ethanol + iodoacetate
-
-
-
?
3.1.1.1
L-Tyr ethyl ester + H2O
-
171016
Rattus norvegicus
L-Tyr + ethanol
-
-
-
?
3.1.1.1
additional information
-
171016
Rattus norvegicus
?
-
-
-
-
3.1.1.1
n-butyl n-butyrate + H2O
-
171016
Rattus norvegicus
n-butanol + n-butyrate
-
-
-
?
3.1.1.1
procaine + H2O
-
171016
Rattus norvegicus
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.1
5.5
10
hydrolysis of methyl butyrate, at optimal methyl butyrate concentrations, 0.01 M
Rattus norvegicus
3.1.1.1
7.5
-
hydrolysis of methyl hutyrate, at high substrate concentrations, 0.5 M
Rattus norvegicus
3.1.1.1
8
8.5
hydrolysis of p-nitrophenyl acetate
Rattus norvegicus