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Literature summary extracted from

  • Saboori, A.M.; Newcombe, D.S.
    Human monocyte carboxylesterase. Purification and kinetics (1990), J. Biol. Chem., 265, 19792-19799.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.1 ethanol activates Homo sapiens
3.1.1.1 ethylene glycol little activating effect, except at high concentrations, 1 M Homo sapiens
3.1.1.1 ethylene glycol monoethyl ether little activating effect, except at high concentrations, 1 M Homo sapiens
3.1.1.1 methanol activates Homo sapiens
3.1.1.1 n-butanol 0.1 M, increases activity Homo sapiens

General Stability

EC Number General Stability Organism
3.1.1.1 dialysis using nitrocellulose membranes causes a significant loss of activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 Dimethylsulfoxide
-
Homo sapiens
3.1.1.1 diphenyl carbonate
-
Homo sapiens
3.1.1.1 n-butanol 1 M Homo sapiens
3.1.1.1 NaF
-
Homo sapiens
3.1.1.1 organophosphate compounds
-
Homo sapiens
3.1.1.1 paraoxon
-
Homo sapiens
3.1.1.1 phenol
-
Homo sapiens
3.1.1.1 PMSF
-
Homo sapiens
3.1.1.1 triphenyl phosphate
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.1
-
2-Nitrophenyl butyrate
-
Homo sapiens
3.1.1.1 0.125
-
4-nitrophenyl pentanoate
-
Homo sapiens
3.1.1.1 0.16
-
1-naphthyl acetate
-
Homo sapiens
3.1.1.1 0.33
-
1-naphthyl butyrate
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 membrane
-
Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 60000
-
3 * 60000, SDS-PAGE Homo sapiens
3.1.1.1 200000
-
gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Homo sapiens
-
at least 4 major isoenzymes in blood monocytes
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.1 side-chain modification contains an N-linked high mannose type carbohydrate chain. Two glucosamine and eight mannose molecules per mol of enzyme Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.1 monocyte at least 4 major isoenzymes Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 additional information
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.1.1 -80°C, 10% glycerol, stable for months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 1-naphthyl acetate + H2O
-
Homo sapiens 1-naphthol + acetate
-
?
3.1.1.1 1-naphthyl butyrate + H2O
-
Homo sapiens 1-naphthol + butyrate
-
?
3.1.1.1 2-nitrophenyl butyrate + H2O
-
Homo sapiens 2-nitrophenol + butyrate
-
?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
Homo sapiens 4-nitrophenol + butyrate
-
?
3.1.1.1 4-nitrophenyl pentanoate + H2O
-
Homo sapiens 4-nitrophenol + pentanoate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 trimer 3 * 60000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 8
-
hydrolysis of 1-naphthyl butyrate Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 6 12 less than 50% of maximal activity at pH less than 6 or more than 12 Homo sapiens