Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bause, E.; Schweden, J.; Gross, A.; Orthen, B.
    Purification and characterization of trimming glucosidase I from pig liver (1989), Eur. J. Biochem., 183, 661-669.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.106 native form of glucosidase I is unusually resistant to non-specific proteolysis, partial digestion with trypsin requires rather high concentrations of the protease, at high, that is equimolar, concentrations of trypsin enzyme is cleaved into a defined and enzymatically active mixture of protein fragments with molecular mass of 69 kDa, 45 kDa and 29 kDa Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.106 1-deoxynojirimycin strong inhibition Bos taurus
3.2.1.106 1-deoxynojirimycin strong inhibition Homo sapiens
3.2.1.106 1-deoxynojirimycin strong inhibition; strong inhibition, Ki = 0.0021 mM Sus scrofa
3.2.1.106 Cu2+
-
 Bos taurus
3.2.1.106 Cu2+ partial inhibition Sus scrofa
3.2.1.106 kojibiose alpha-1,2-linked glucose disaccharide Bos taurus
3.2.1.106 kojibiose alpha-1,2-linked glucose disaccharide; Ki = 0.095 mM Sus scrofa
3.2.1.106 additional information
-
 Bos taurus
3.2.1.106 additional information
-
 Homo sapiens
3.2.1.106 additional information no inhibition by 1 mM EDTA or 1 mM o-phenanthroline Sus scrofa
3.2.1.106 N,N-dimethyl-1-deoxynojirimycin strong inhibition Bos taurus
3.2.1.106 N,N-dimethyl-1-deoxynojirimycin strong inhibition Homo sapiens
3.2.1.106 N,N-dimethyl-1-deoxynojirimycin strong inhibition; strong inhibition, Ki = 0.0005 mM Sus scrofa
3.2.1.106 N-(5-carboxypentyl)-1-deoxynojirimycin strong inhibition Bos taurus
3.2.1.106 N-(5-carboxypentyl)-1-deoxynojirimycin strong inhibition; strong specific inhibition, Ki = 0.00045 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.106 additional information
-
 additional information kinetic data Sus scrofa
3.2.1.106 additional information
-
 additional information kinetic data Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.106 endoplasmic reticulum endoplasmic reticulum-resident glycoprotein Homo sapiens 5783
-
3.2.1.106 endoplasmic reticulum endoplasmic reticulum-resident glycoprotein Sus scrofa 5783
-
3.2.1.106 endoplasmic reticulum rough endoplasmic reticulum Sus scrofa 5783
-
3.2.1.106 endoplasmic reticulum rough endoplasmic reticulum Bos taurus 5783
-
3.2.1.106 membrane membrane-bound Homo sapiens 16020
-
3.2.1.106 membrane membrane-bound Sus scrofa 16020
-
3.2.1.106 membrane membrane-bound Bos taurus 16020
-
3.2.1.106 microsome
-
 Homo sapiens
-
-
3.2.1.106 microsome
-
 Sus scrofa
-
-
3.2.1.106 microsome
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.106 additional information metal ions not required Homo sapiens
3.2.1.106 additional information metal ions not required Sus scrofa
3.2.1.106 additional information metal ions not required Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.106 85000
-
 SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O Homo sapiens hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Sus scrofa hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Bos taurus hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Homo sapiens specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Sus scrofa specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Bos taurus specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Homo sapiens involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Bos taurus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Homo sapiens involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Bos taurus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Homo sapiens involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Bos taurus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Homo sapiens involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Bos taurus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Sus scrofa glucose processing is essential for pro-receptor transport and maturation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Bos taurus
-
 calf
-
3.2.1.106 Homo sapiens
-
-
-
3.2.1.106 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.106 glycoprotein with high-mannose oligosaccharides Homo sapiens
3.2.1.106 glycoprotein with high-mannose oligosaccharides Sus scrofa
3.2.1.106 glycoprotein with high-mannose oligosaccharides Bos taurus
3.2.1.106 glycoprotein N-glycoprotein, one N-linked high-mannose sugar chain of less than 3 kDA Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.106
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.106 intestine calf Bos taurus
-
3.2.1.106 kidney calf Bos taurus
-
3.2.1.106 liver
-
 Homo sapiens
-
3.2.1.106 liver
-
 Sus scrofa
-
3.2.1.106 liver calf Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.106 additional information
-
-
 Sus scrofa
3.2.1.106 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.2.1.106 4°C, stable for several weeks Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Homo sapiens D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Sus scrofa D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Homo sapiens ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Sus scrofa ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Homo sapiens ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Sus scrofa ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Bos taurus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Homo sapiens ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Bos taurus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Homo sapiens ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Bos taurus ?
-
 ?
3.2.1.106 additional information Glc1Man9GlcNAc2: not a substrate Homo sapiens ?
-
 ?
3.2.1.106 additional information Glc1Man9GlcNAc2: not a substrate Sus scrofa ?
-
 ?
3.2.1.106 additional information Glc1Man9GlcNAc2: not a substrate Bos taurus ?
-
 ?
3.2.1.106 additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Homo sapiens ?
-
 ?
3.2.1.106 additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Sus scrofa ?
-
 ?
3.2.1.106 additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Bos taurus ?
-
 ?
3.2.1.106 additional information methylumbelliferyl-beta-glucosides: no substrates Sus scrofa ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Homo sapiens ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Sus scrofa ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Bos taurus ?
-
 ?
3.2.1.106 additional information Glc2Man9GlcNAc2: not a substrate Homo sapiens ?
-
 ?
3.2.1.106 additional information Glc2Man9GlcNAc2: not a substrate Sus scrofa ?
-
 ?
3.2.1.106 additional information Glc2Man9GlcNAc2: not a substrate Bos taurus ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-beta-D-glucopyranoside: not a substrate Sus scrofa ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-beta-D-glucopyranoside: not a substrate Bos taurus ?
-
 ?
3.2.1.106 additional information substrate specificities Sus scrofa ?
-
 ?
3.2.1.106 additional information substrate specificities Bos taurus ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Homo sapiens ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Bos taurus ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Homo sapiens ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Bos taurus ?
-
 ?
3.2.1.106 additional information glucose processing is essential for pro-receptor transport and maturation Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.106 ?
-
 Bos taurus
3.2.1.106 ? x * 85000, SDS-PAGE, reducing conditions Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.106 37
-
 assay at Sus scrofa
3.2.1.106 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.106 6.4
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.106 5.6 7.3 half-maximal activity at, steep decrease in activity on acidic side Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.106 0.00045
-
 N-(5-carboxypentyl)-1-deoxynojirimycin strong specific inhibition Sus scrofa
3.2.1.106 0.0005
-
 N,N-dimethyl-1-deoxynojirimycin strong inhibition Sus scrofa