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Literature summary extracted from

  • Kilker, R.D.Jr.; Saunier, B.; Tkacz, J.S.; Herscovics, A.
    Partial purification from Saccharomyces cerevisiae of a soluble glucosidase which removes the terminal glucose from the oligosaccharide Glc3Man9GlcNAc2 (1981), J. Biol. Chem., 256, 5299-5303.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.106 Triton X-100 about 45% activation of membrane-bound activity, maximum stimulation of membrane-bound activity at 0.5% Triton X-100, no activation of soluble activity Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
3.2.1.106 bovine serum albumin, 1-8 mg/ml, enhances stability during assay Saccharomyces cerevisiae
3.2.1.106 quite stable at all stages of purification Saccharomyces cerevisiae
3.2.1.106 stable to lyophilization without dialysis after step 3 of purification Saccharomyces cerevisiae
3.2.1.106 unstable to dialysis against, 10 mM potassium phosphate buffer, pH 6.8, containing 0.002% Hibitane, using either dialysis bags or diafiltration Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.106 additional information no inhibition by dithiothreitol, 2-mercaptoethanol, Mg2+, Mn2+, EDTA, potassium phosphate Saccharomyces cerevisiae
3.2.1.106 octyl-beta-glucoside 10 mM: 90% inhibition Saccharomyces cerevisiae
3.2.1.106 p-Aminophenyl-beta-thioglucoside 100 mM: 73% inhibition Saccharomyces cerevisiae
3.2.1.106 Tris/maleate 10 mM: 60% inhibition Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.106 membrane X-2180, equally distributed between particulate and supernatant fractions Saccharomyces cerevisiae 16020
-
3.2.1.106 additional information X-2180, equally distributed between particulate and supernatant fractions Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.106 additional information no requirement for divalent cations Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O Saccharomyces cerevisiae hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Saccharomyces cerevisiae specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Saccharomyces cerevisiae involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Saccharomyces cerevisiae involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Saccharomyces cerevisiae involved in the formation of high mannose and complex glycoproteins ?
-
 ?
3.2.1.106 additional information Saccharomyces cerevisiae X-2180 involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Saccharomyces cerevisiae
-
 X-2180
-
3.2.1.106 Saccharomyces cerevisiae X-2180
-
 X-2180
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.106 partial, soluble glucosidase I Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.106 additional information
-
-
 Saccharomyces cerevisiae
3.2.1.106 2.6
-
 soluble activity Saccharomyces cerevisiae
3.2.1.106 5.5
-
 membrane-bound activity, with Triton X-100 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.2.1.106 -26°C, lyophilized, stable for at least 1 month Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Saccharomyces cerevisiae D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information Glc1Man9GlcNAc2: not a substrate Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information very slightly active with Glc2Man9GlcNAc2 Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information involved in the formation of high mannose and complex glycoproteins Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information Glc1Man9GlcNAc2: not a substrate Saccharomyces cerevisiae X-2180 ?
-
 ?
3.2.1.106 additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Saccharomyces cerevisiae X-2180 ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Saccharomyces cerevisiae X-2180 ?
-
 ?
3.2.1.106 additional information very slightly active with Glc2Man9GlcNAc2 Saccharomyces cerevisiae X-2180 ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Saccharomyces cerevisiae X-2180 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.106 6.8
-
 rapid decrease in activity on acidic side Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.106 6 8.5 pH 6.0: about 35% of maximal activity, pH 8.5: about 15% of maximal activity, pH 5.8: no activity Saccharomyces cerevisiae