BRENDA - Enzyme Database

The purification and properties of glutamine-dependent asparagine synthetase isolated from Lupinus luteus

Lea, P.J.; Fowden, L.; Proc. R. Soc. Lond. B Biol. Sci. 192, 13-26 (1975)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
6.3.5.4
glycerol and 2-mercaptoethanol are essential for enzyme stability during purification at 4°C
Lupinus albus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.4
2-Amino-2-carboxy-L-ethanesulfonamide
-
Lupinus albus
6.3.5.4
2-Hydroxyethyl-L-Gln
-
Lupinus albus
6.3.5.4
2-oxoglutarate
in presence of aminoxyacetate, 50% inhibition at 5 mM
Lupinus albus
6.3.5.4
5-Bromo-4-oxo-L-norvaline
-
Lupinus albus
6.3.5.4
5-Chloro-4-oxo-L-norvaline
-
Lupinus albus
6.3.5.4
Albizzine
-
Lupinus albus
6.3.5.4
AMP
2.5 mM, 50% inhibition
Lupinus albus
6.3.5.4
Asn
-
Lupinus albus
6.3.5.4
ATP
strong inhibition above 5 mM
Lupinus albus
6.3.5.4
azaserine
-
Lupinus albus
6.3.5.4
beta-Methyl-DL-Asn
-
Lupinus albus
6.3.5.4
Ca2+
-
Lupinus albus
6.3.5.4
Cu2+
-
Lupinus albus
6.3.5.4
diphosphate
5 mM, 45% inhibition
Lupinus albus
6.3.5.4
DL-homo-Gln
-
Lupinus albus
6.3.5.4
erythro-beta-Hydroxy-L-Asn
-
Lupinus albus
6.3.5.4
erythro-beta-hydroxy-L-Asp
-
Lupinus albus
6.3.5.4
erythro-beta-Methyl-L-Asp
-
Lupinus albus
6.3.5.4
gamma-Methylene-L-Gln
-
Lupinus albus
6.3.5.4
Gln
D-Gln, weak
Lupinus albus
6.3.5.4
Glu
150 mM, 50% inhibition
Lupinus albus
6.3.5.4
L-Glutamate diamide
-
Lupinus albus
6.3.5.4
L-Glutamate-gamma-ethyl ester
-
Lupinus albus
6.3.5.4
L-glutamate-gamma-methyl ester
-
Lupinus albus
6.3.5.4
L-methionine-S-sulfoximine
-
Lupinus albus
6.3.5.4
Li+
-
Lupinus albus
6.3.5.4
meso-Diaminosuccinamate
-
Lupinus albus
6.3.5.4
N-acetyl-L-Gln
-
Lupinus albus
6.3.5.4
N-alpha-Methyl-L-Asn
-
Lupinus albus
6.3.5.4
N-Carbobenzoxy-DL-Gln
-
Lupinus albus
6.3.5.4
N-ethyl-L-Asn
-
Lupinus albus
6.3.5.4
N-Methyl-L-Asn
-
Lupinus albus
6.3.5.4
S-Carbamoylcysteine
-
Lupinus albus
6.3.5.4
Sn2+
-
Lupinus albus
6.3.5.4
Sr2+
-
Lupinus albus
6.3.5.4
threo-beta-Hydroxy-L-Asn
-
Lupinus albus
6.3.5.4
threo-beta-methyl-L-Asp
-
Lupinus albus
6.3.5.4
Zn2+
-
Lupinus albus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.4
0.04
-
L-Gln
-
Lupinus albus
6.3.5.4
0.15
-
ATP
-
Lupinus albus
6.3.5.4
0.8
-
L-Asp
-
Lupinus albus
6.3.5.4
2.1
-
NH4+
-
Lupinus albus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.3.5.4
cytosol
-
Lupinus albus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.5.4
Co2+
can partially replace Mg2+ in activation
Lupinus albus
6.3.5.4
Mg2+
Km: 1.1 mM
Lupinus albus
6.3.5.4
Mn2+
can partially substitute for Mg2+
Lupinus albus
6.3.5.4
Ni2+
can partially replace Mg2+ in activation
Lupinus albus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.5.4
Lupinus albus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.5.4
cotyledon
-
Lupinus albus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.5.4
0.193
-
-
Lupinus albus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.4
ATP + L-Asp + L-Gln
-
1709
Lupinus albus
AMP + diphosphate + Asn + Glu
-
-
-
-
6.3.5.4
ATP + L-Asp + NH3
30% of the activity relative to Gln
1709
Lupinus albus
AMP + diphosphate + Asn
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.5.4
7.9
8.3
-
Lupinus albus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.3.5.4
2.5
-
AMP
-
Lupinus albus
6.3.5.4
150
-
Glu
-
Lupinus albus
General Stability (protein specific)
EC Number
General Stability
Organism
6.3.5.4
glycerol and 2-mercaptoethanol are essential for enzyme stability during purification at 4°C
Lupinus albus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.4
2-Amino-2-carboxy-L-ethanesulfonamide
-
Lupinus albus
6.3.5.4
2-Hydroxyethyl-L-Gln
-
Lupinus albus
6.3.5.4
2-oxoglutarate
in presence of aminoxyacetate, 50% inhibition at 5 mM
Lupinus albus
6.3.5.4
5-Bromo-4-oxo-L-norvaline
-
Lupinus albus
6.3.5.4
5-Chloro-4-oxo-L-norvaline
-
Lupinus albus
6.3.5.4
Albizzine
-
Lupinus albus
6.3.5.4
AMP
2.5 mM, 50% inhibition
Lupinus albus
6.3.5.4
Asn
-
Lupinus albus
6.3.5.4
ATP
strong inhibition above 5 mM
Lupinus albus
6.3.5.4
azaserine
-
Lupinus albus
6.3.5.4
beta-Methyl-DL-Asn
-
Lupinus albus
6.3.5.4
Ca2+
-
Lupinus albus
6.3.5.4
Cu2+
-
Lupinus albus
6.3.5.4
diphosphate
5 mM, 45% inhibition
Lupinus albus
6.3.5.4
DL-homo-Gln
-
Lupinus albus
6.3.5.4
erythro-beta-Hydroxy-L-Asn
-
Lupinus albus
6.3.5.4
erythro-beta-hydroxy-L-Asp
-
Lupinus albus
6.3.5.4
erythro-beta-Methyl-L-Asp
-
Lupinus albus
6.3.5.4
gamma-Methylene-L-Gln
-
Lupinus albus
6.3.5.4
Gln
D-Gln, weak
Lupinus albus
6.3.5.4
Glu
150 mM, 50% inhibition
Lupinus albus
6.3.5.4
L-Glutamate diamide
-
Lupinus albus
6.3.5.4
L-Glutamate-gamma-ethyl ester
-
Lupinus albus
6.3.5.4
L-glutamate-gamma-methyl ester
-
Lupinus albus
6.3.5.4
L-methionine-S-sulfoximine
-
Lupinus albus
6.3.5.4
Li+
-
Lupinus albus
6.3.5.4
meso-Diaminosuccinamate
-
Lupinus albus
6.3.5.4
N-acetyl-L-Gln
-
Lupinus albus
6.3.5.4
N-alpha-Methyl-L-Asn
-
Lupinus albus
6.3.5.4
N-Carbobenzoxy-DL-Gln
-
Lupinus albus
6.3.5.4
N-ethyl-L-Asn
-
Lupinus albus
6.3.5.4
N-Methyl-L-Asn
-
Lupinus albus
6.3.5.4
S-Carbamoylcysteine
-
Lupinus albus
6.3.5.4
Sn2+
-
Lupinus albus
6.3.5.4
Sr2+
-
Lupinus albus
6.3.5.4
threo-beta-Hydroxy-L-Asn
-
Lupinus albus
6.3.5.4
threo-beta-methyl-L-Asp
-
Lupinus albus
6.3.5.4
Zn2+
-
Lupinus albus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.3.5.4
2.5
-
AMP
-
Lupinus albus
6.3.5.4
150
-
Glu
-
Lupinus albus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.4
0.04
-
L-Gln
-
Lupinus albus
6.3.5.4
0.15
-
ATP
-
Lupinus albus
6.3.5.4
0.8
-
L-Asp
-
Lupinus albus
6.3.5.4
2.1
-
NH4+
-
Lupinus albus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.3.5.4
cytosol
-
Lupinus albus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.5.4
Co2+
can partially replace Mg2+ in activation
Lupinus albus
6.3.5.4
Mg2+
Km: 1.1 mM
Lupinus albus
6.3.5.4
Mn2+
can partially substitute for Mg2+
Lupinus albus
6.3.5.4
Ni2+
can partially replace Mg2+ in activation
Lupinus albus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.5.4
cotyledon
-
Lupinus albus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.5.4
0.193
-
-
Lupinus albus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.4
ATP + L-Asp + L-Gln
-
1709
Lupinus albus
AMP + diphosphate + Asn + Glu
-
-
-
-
6.3.5.4
ATP + L-Asp + NH3
30% of the activity relative to Gln
1709
Lupinus albus
AMP + diphosphate + Asn
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.5.4
7.9
8.3
-
Lupinus albus