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Literature summary extracted from

  • Shyur, L.F.; Poland, B.W.; Honzatko, R.B.; Fromm, H.J.
    Major changes in the kinetic mechanism of AMP inhibition and AMP cooperativity attends the mutation of Arg49 in fructose-1,6-bisphosphatase (1997), J. Biol. Chem., 272, 26295-26299.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.11 R49C less thermostable than wild type enzyme, wild type values for turnover number and Km-value Sus scrofa
3.1.3.11 R49D less thermostable than wild type enzyme, wild type values for turnover number and Km-value, increased inhibition constant for fructose 2,6-diphosphate. Mechanism of AMP inhibition with respect to fructose 1,6-diphosphate changes from noncompetitive, wild type, to competitive. Mechanism of AMP inhibition with respect to fructose 1,6-diphosphate changes from noncompetitive, wild type, to uncompetitive. Loss of AMP cooperativity Sus scrofa
3.1.3.11 R49L less thermostable than wild type enzyme, wild type values for turnover number and Km-value, increased inhibition constant for fructose 2,6-diphosphate. Mechanism of AMP inhibition with respect to fructose 1,6-diphosphate changes from noncompetitive, wild type, to competitive. Loss of AMP cooperativity Sus scrofa
3.1.3.11 R49M enzyme is more thermostable than wild type enzyme, kinetic properties are similar to the wild type enzyme. Loss of AMP cooperativity Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.00344
-
 fructose 1,6-diphosphate mutant enzyme R49L Sus scrofa
3.1.3.11 0.00351
-
 fructose 1,6-diphosphate wild type enzyme Sus scrofa
3.1.3.11 0.00402
-
 fructose 1,6-diphosphate mutant enzyme K50M Sus scrofa
3.1.3.11 0.00407
-
 fructose 1,6-diphosphate mutant enzyme R49C Sus scrofa
3.1.3.11 0.00442
-
 fructose 1,6-diphosphate mutant enzyme R49D Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.11 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
 Sus scrofa D-fructose 6-phosphate + phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 18
-
 fructose 1,6-diphosphate wild type enzyme Sus scrofa
3.1.3.11 20.1
-
 fructose 1,6-diphosphate mutant enzyme R49D Sus scrofa
3.1.3.11 22.4
-
 fructose 1,6-diphosphate mutant enzyme K50M Sus scrofa
3.1.3.11 23.6
-
 fructose 1,6-diphosphate mutant enzyme R49L and R49C Sus scrofa