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Literature summary extracted from

  • Springgate, C.F.; Stachow, C.S.
    Fructose 1,6-diphosphatase from Rhodopseudomonas palustris. I. Purification and properties (1972), Arch. Biochem. Biophys., 152, 1-12.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.11 Cys stimulates Rhodopseudomonas palustris
3.1.3.11 DTT activates Rhodopseudomonas palustris
3.1.3.11 GSH activates Rhodopseudomonas palustris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 1,10-phenanthroline
-
 Rhodopseudomonas palustris
3.1.3.11 8-hydroxyquinoline
-
 Rhodopseudomonas palustris
3.1.3.11 Cs+
-
 Rhodopseudomonas palustris
3.1.3.11 EDTA 0.1 mM, complete inhibition Rhodopseudomonas palustris
3.1.3.11 K+
-
 Rhodopseudomonas palustris
3.1.3.11 KCN
-
 Rhodopseudomonas palustris
3.1.3.11 Li+
-
 Rhodopseudomonas palustris
3.1.3.11 Na+
-
 Rhodopseudomonas palustris
3.1.3.11 NH4+
-
 Rhodopseudomonas palustris
3.1.3.11 p-hydroxymercuribenzoate
-
 Rhodopseudomonas palustris
3.1.3.11 sedoheptulose 1,7-diphosphate competitive inhibition of activity with fructose 1,6-diphosphate Rhodopseudomonas palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.045
-
 fructose 1,6-diphosphate
-
 Rhodopseudomonas palustris
3.1.3.11 0.3
-
 sedoheptulose 1,7-diphosphate
-
 Rhodopseudomonas palustris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ only 27% of the maximal activity observed with Mn2+ Rhodopseudomonas palustris
3.1.3.11 Mg2+ Km: 3.3 mM Rhodopseudomonas palustris
3.1.3.11 Mg2+ divalent cation required. Mn2+ or Mg2+ Rhodopseudomonas palustris
3.1.3.11 Mn2+ divalent cation required. Mn2+ or Mg2+ Rhodopseudomonas palustris
3.1.3.11 Mn2+ Km: 0.022 mM Rhodopseudomonas palustris
3.1.3.11 Mn2+ Km: 0.078 mM Rhodopseudomonas palustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 130000
-
 sucrose density gradient centrifugation Rhodopseudomonas palustris

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 8.81
-
-
 Rhodopseudomonas palustris

Storage Stability

EC Number Storage Stability Organism
3.1.3.11 -20°C, 0.1 mM MnCl2, 1 mM DTT, 0.01 M Tris-HCl buffer, pH 7.4, stable for at least 3 months Rhodopseudomonas palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-diphosphate + H2O
-
 Rhodopseudomonas palustris D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 sedoheptulose 1,7-bisphosphate + H2O at 22.4% of the activity with fructose 1,6-diphosphate Rhodopseudomonas palustris sedoheptulose 7-phosphate + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.11 50
-
 1 min, 65% loss of activity without Mn2+, 12% loss of activity in presence of 0.3 mM Mn2+ Rhodopseudomonas palustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8.5
-
 hydrolysis of fructose 1,6-diphosphate or sedoheptulose 1,7-diphosphate Rhodopseudomonas palustris