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Literature summary extracted from

  • Riazuddin, S.; Grossman, L.
    Micrococcus luteus correndonucleases. I. resolution and purification of two endonucleases specific for DNA containing pyrimidine dimers (1977), J. Biol. Chem., 252, 6280-6286.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.25.1 rATP stimulates Py-Py correndonuclease I activity Micrococcus luteus
3.1.25.1 Tris-HCl optimum for Py-Py correndonuclease I: 10 mM supplemented with 50 mM NaCl or potassium phosphate Micrococcus luteus
3.1.25.1 Tris-HCl optimum for Py-Py correndonuclease II: 10 mM Micrococcus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.25.1 Ca2+ inhibits both Py-Py correndonucleases Micrococcus luteus
3.1.25.1 EDTA inhibits Py-Py correndonuclease II at concentrations above 20 mM Micrococcus luteus
3.1.25.1 KCl Py-Py correndonuclease II loses 75% of activity in presence of 100 mM Micrococcus luteus
3.1.25.1 Mg2+ Py-Py correndonuclease II loses 75% of activity in presence of 15 mM Micrococcus luteus
3.1.25.1 NaCl Py-Py correndonuclease II loses 75% of activity in presence of 100 mM Micrococcus luteus
3.1.25.1 Zn2+ inhibits both Py-Py correndonucleases Micrococcus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.25.1 NaCl activating Micrococcus luteus
3.1.25.1 NaCl optimum for Py-Py correndonuclease I: 50 mM supplemented with 10 mM Tris-HCl Micrococcus luteus
3.1.25.1 potassium phosphate activating, optimum for Py-Py correndonuclease I: 50 mM supplemented with 10 mM Tris-HCl Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.25.1 11000 15000 Py-Py correndonucleases I and II Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.25.1 DNA + H2O Micrococcus luteus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.25.1 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.25.1
-
 Micrococcus luteus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.25.1 additional information
-
-
 Micrococcus luteus

Storage Stability

EC Number Storage Stability Organism
3.1.25.1 -20°C, in 50% glycerol, complete inactivation in 3-4 weeks Micrococcus luteus
3.1.25.1 in liquid nitrogen, for minimal inactivation Micrococcus luteus
3.1.25.1 in liquid nitrogen, for minimal inactivation, freeze-thawing does not inactivate either Py-Py correndonuclease Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.25.1 DNA + H2O
-
 Micrococcus luteus ?
-
 ?
3.1.25.1 additional information not: x-irradiated, psoralen-crosslinked or alkylated DNA Micrococcus luteus ?
-
 ?
3.1.25.1 X174 DNA + H2O UV-irradiated, denatured DNA: substrate for Py-Py correndonuclease I only Micrococcus luteus RFII
-
 ?
3.1.25.1 X174 DNA + H2O UV irradiated RFI of phage X174 containing apurinic sites Micrococcus luteus RFII
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.25.1 55
-
 Py-Py correndonuclease I loses 20% of activity after 5 min, Py-Py correndonuclease II loses more than 75% of activity after 5 min Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.25.1 7 7.4 Py-Py correndonucleases I and II Micrococcus luteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.25.1 4 10 Py-Py correndonucleases I and II, no activity below or above these values Micrococcus luteus