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Literature summary extracted from

  • Politino, M.; Tonzi, S.M.; Burnett, W.V.; Romancik, G.; Usher, J.J.
    Purification and characterization of a cephalosporin esterase from Phodosporidium toruloides (1997), Appl. Environ. Microbiol., 63, 4807-4811.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.1.1.41 unstable after deglycosylation with endoglycosidase H Rhodotorula toruloides

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.41 3,4-dichloroisocoumarin
-
 Rhodotorula toruloides
3.1.1.41 dimethyl phosphite
-
 Rhodotorula toruloides
3.1.1.41 PMSF
-
 Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.41 51.8
-
 cephalosporin C
-
 Rhodotorula toruloides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.41 61315
-
 x * 61315, calculation from amino acid sequence Rhodotorula toruloides
3.1.1.41 80000
-
 x * 80000, SDS-PAGE Rhodotorula toruloides

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Bacillus subtilis
-
-
-
3.1.1.41 Rhodotorula toruloides
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.41 side-chain modification glycoprotein, sugar moieties may play a role in stabilizing the native structure of the enzyme Rhodotorula toruloides

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.41
-
 Rhodotorula toruloides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.41 additional information
-
-
 Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 cephalosporin C + H2O
-
 Bacillus subtilis deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 cephalosporin C + H2O
-
 Rhodotorula toruloides deacetylcephalosporin C + acetate
-
 ?
3.1.1.41 deacetyl-7-aminocephalosporanic acid + ethylene glycol diacetate
-
 Bacillus subtilis 7-aminocephalosporanic acid + ?
-
 ?
3.1.1.41 deacetyl-7-aminocephalosporanic acid + ethylene glycol diacetate
-
 Rhodotorula toruloides 7-aminocephalosporanic acid + ?
-
 ?
3.1.1.41 deacetyl-7-aminocephalosporanic acid + isopropenyl acetate
-
 Bacillus subtilis 7-aminocephalosporanic acid + ?
-
 ?
3.1.1.41 p-nitrophenyl acetate + H2O
-
 Bacillus subtilis 4-nitrophenol + acetate
-
 ?
3.1.1.41 p-nitrophenyl acetate + H2O
-
 Rhodotorula toruloides 4-nitrophenol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.41 ? x * 80000, SDS-PAGE Rhodotorula toruloides
3.1.1.41 ? x * 61315, calculation from amino acid sequence Rhodotorula toruloides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.41 25
-
-
 Rhodotorula toruloides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.41 25
-
-
 Bacillus subtilis
3.1.1.41 25
-
 unstable above Rhodotorula toruloides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.41 6
-
-
 Rhodotorula toruloides