Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Boehlein, S.K.; Schuster, S.M.; Richards, N.G.J.
    Glutamic acid gamma-monohydroxylamine are alternate substrates for Escherichia coli asparagine synthetase B (1996), Biochemistry, 35, 3031-3037.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.4 N74A mutant N74A, in which Asn is replaced by Ala can also use NH2OH as an alternative substrate to NH4+ and catalyze the hydrolysis of L-glutamic acid gamma-monohydroxamate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.4 0.04
-
 L-Gln mutant N74A Escherichia coli
6.3.5.4 0.09
-
 L-glutamic acid gamma-monohydroxamate mutant N74A Escherichia coli
6.3.5.4 0.26
-
 L-glutamic acid gamma-monohydroxamate wild-type enzyme Escherichia coli
6.3.5.4 0.69
-
 L-Gln wild-type enzyme Escherichia coli
6.3.5.4 5.24
-
 Gln mutant C99A Escherichia coli
6.3.5.4 11.5
-
 hydroxylamine wild-type enzyme Escherichia coli
6.3.5.4 13
-
 NH4+ mutant N74A Escherichia coli
6.3.5.4 15.7
-
 NH4+ wild-type enzyme Escherichia coli
6.3.5.4 17.1
-
 hydroxylamine mutant N74A Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Escherichia coli
-
 wild-type and mutant N74A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-Asp + L-Gln
-
 Escherichia coli AMP + diphosphate + Asn + Glu
-
 ?
6.3.5.4 ATP + L-Asp + NH2OH
-
 Escherichia coli AMP + diphosphate + beta-aspartylhydroxamate
-
 ?
6.3.5.4 L-Glutamic acid gamma-monohydroxamate + H2O
-
 Escherichia coli Hydroxylamine + Glu
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.4 0.05
-
 L-Gln mutant N74A Escherichia coli
6.3.5.4 0.1
-
 L-glutamic acid gamma-monohydroxamate mutant N74A Escherichia coli
6.3.5.4 0.15
-
 L-glutamic acid gamma-monohydroxamate wild-type Escherichia coli
6.3.5.4 0.59
-
 NH4+ wild-type Escherichia coli
6.3.5.4 0.65
-
 NH4+ mutant N74A Escherichia coli
6.3.5.4 1.01
-
 L-Gln wild-type enzyme Escherichia coli
6.3.5.4 1.03
-
 hydroxylamine wild-type enzyme Escherichia coli
6.3.5.4 1.17
-
 hydroxylamine N74A mutant Escherichia coli