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Literature summary extracted from

  • Buchanan, J.M.
    The Amidotransferases (1973), Adv. Enzymol. Relat. Areas Mol. Biol., 39, 91-183.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.5.3 K+ required for stabilization Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.3 6-diazo-5-oxo-L-norleucine
-
 Gallus gallus
6.3.5.3 ADP
-
 Gallus gallus
6.3.5.3 albizziin presence of beta-5'-phosphoribosylformylglycinamide, ATP, and Mg2+ inhibits inactivation Gallus gallus
6.3.5.3 beta-gamma-5'-Adenylyl methylene diphosphate
-
 Gallus gallus
6.3.5.3 Cyanate
-
 Gallus gallus
6.3.5.3 hydroxylamine
-
 Gallus gallus
6.3.5.3 iodoacetamide rate of inactivation is 20-30times greater in the presence than in the absence of beta-5'-phosphoribosylformylglycinamide, ATP, and Mg2+ Gallus gallus
6.3.5.3 iodoacetate rate of inactivation is 20-30times greater in the presence than in the absence of beta-5'-phosphoribosylformylglycinamide, ATP, and Mg2+ Gallus gallus
6.3.5.3 L-2-Amino-4-oxo-5-chloropentanoic acid
-
 Gallus gallus
6.3.5.3 Na+
-
 Gallus gallus
6.3.5.3 O-diazoacetyl-L-Ser
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.3 additional information
-
 additional information
-
 Gallus gallus
6.3.5.3 additional information
-
 additional information
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.3 Co2+ can replace Mg2+ in activation Gallus gallus
6.3.5.3 K+
-
 Mus musculus
6.3.5.3 K+ enhances the rate of reaction by 20% up to a concentration of 0.1 M, gradual inhibition at higher concentrations Gallus gallus
6.3.5.3 Mg2+ required Gallus gallus
6.3.5.3 Mn2+ can replace Mg2+ in activation Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.3 133000
-
 1 * 133000, SDS-PAGE. When stored in absence of a reducing agent, e.g. DTT or 2-mercaptoethanol, the enzyme undergoes polymerization Gallus gallus
6.3.5.3 133000 135000
-
 Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 133000 135000
-
 Gallus gallus
6.3.5.3 133000 135000
-
 Pigeon

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln Gallus gallus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.3 Gallus gallus
-
-
-
6.3.5.3 Mus musculus
-
-
-
6.3.5.3 Pigeon
-
-
-
6.3.5.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.5.3 ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate ping-pong mechanism Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.3 Ehrlich ascites carcinoma cell
-
 Mus musculus
-
6.3.5.3 liver
-
 Gallus gallus
-
6.3.5.3 liver
-
 Pigeon
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln
-
 Gallus gallus ?
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
 Gallus gallus ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
 Mus musculus ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O
-
 Pigeon ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O
-
 Gallus gallus ADP + phosphate + 5'-phosphoribosylformylglycinamidine
-
 ?
6.3.5.3 ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O
-
 Mus musculus ADP + phosphate + 5'-phosphoribosylformylglycinamidine
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.3 monomer 1 * 133000, SDS-PAGE. When stored in absence of a reducing agent, e.g. DTT or 2-mercaptoethanol, the enzyme undergoes polymerization Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.3 8
-
-
 Gallus gallus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.5.3 6.5
-
 highest stability, rather unstable at pH 8 Gallus gallus