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Literature summary extracted from
- A highly thermostable proline:tRNA ligase from Thermus aquaticus. Purification and enzyme-tRNA recognition at elevated temperatures (1984), Can. J. Biochem. Cell Biol., 62, 507-515.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.15 | 62000 | - |
2 * 62000, SDS-PAGE | Thermus aquaticus |
| 6.1.1.15 | 127000 | - |
gel filtration | Thermus aquaticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.1.1.15 | Thermus aquaticus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.1.1.15 | - |
Thermus aquaticus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.15 | additional information | - |
- |
Thermus aquaticus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 6.1.1.15 | -20°C, pH 6.8, 40% v/v glycerol, enzyme concentrated by ultrafiltration | Thermus aquaticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.1.1.15 | ATP + L-proline + tRNAPro | - |
Thermus aquaticus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.1.1.15 | dimer | 2 * 62000, SDS-PAGE | Thermus aquaticus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.15 | additional information | - |
even at 85°C the enzyme retains its specificity towards its cognate substrate | Thermus aquaticus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.15 | 90 | - |
pH 6-7.8, 30 min, enzyme retains considerable activity | Thermus aquaticus |
| 6.1.1.15 | 95 | - |
pH 6-7.8, 30 min, unstable | Thermus aquaticus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.15 | 7 | - |
Escherichia coli tRNAPro | Thermus aquaticus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.15 | 6 | 9 | pH 6.0: about 30% of maximal activity, pH 9.0: about 40% of maximal activity | Thermus aquaticus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.1.1.15 | 6 | 7.8 | 90°C, 30 min, enzyme contains considerable activity, no activity retained after exposure to 95°C | Thermus aquaticus |
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