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Literature summary extracted from

  • Shenoy, B.C.; Wood, H.G.
    Purification and properties of the synthetase catalyzing the biotination of the aposubunit of transcarboxylase from Propionibacterium shermanii (1988), FASEB J., 2, 2396-2401.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.9 divalent metal is required for storage stability, Mg2+, Mn2+, Co2+, Zn2+, or Ni2+ Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 loses activity by repeated freezing and thawing Propionibacterium freudenreichii subsp. shermanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.9 Co2+ activates, inhibition at high concentrations Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Cu2+
-
 Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 EDTA
-
 Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Hg2+
-
 Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Zn2+ activates, inhibition at high concentrations Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.9 0.0009
-
 1.3 S subunit of apotranscarboxylase
-
 Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 0.038
-
 ATP
-
 Propionibacterium freudenreichii subsp. shermanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.9 Co2+ affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+, Km: 0.0017 mM Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Mg2+ divalent metal ion required Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Mg2+ affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+ Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Mg2+ Km: 0.0019 mM Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Mn2+ affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+ Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Mn2+ Km: 0.0015 mM Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Ni2+ affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+, Km: 0.0013 mM Propionibacterium freudenreichii subsp. shermanii
6.3.4.9 Zn2+ affinity of the enzyme towards metal in decreasing order: Zn2+, Ni2+, Mn2+, Co2+, Mg2+, Km: 0.0003 mM Propionibacterium freudenreichii subsp. shermanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.9 30000
-
 gel filtration Propionibacterium freudenreichii subsp. shermanii

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.9 Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.9
-
 Propionibacterium freudenreichii subsp. shermanii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.9 0.0124
-
-
 Propionibacterium freudenreichii subsp. shermanii

Storage Stability

EC Number Storage Stability Organism
6.3.4.9 -20°C, stable for 6 months Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.9 ATP + biotin + 1.3 S subunit of apotranscarboxylase
-
 Propionibacterium freudenreichii subsp. shermanii ?
-
 ?
6.3.4.9 ATP + biotin + apo-[methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 Propionibacterium freudenreichii subsp. shermanii AMP + diphosphate + [methylmalonyl-CoA:pyruvate carboxyltransferase]
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.9 monomer 1 * 30000, SDS-PAGE Propionibacterium freudenreichii subsp. shermanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.9 37
-
-
 Propionibacterium freudenreichii subsp. shermanii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.4.9 20 60 about 30% of maximal activity at 20°C and 60°C Propionibacterium freudenreichii subsp. shermanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.9 6
-
-
 Propionibacterium freudenreichii subsp. shermanii