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Literature summary extracted from

  • Takada, S.; Kusumi, T.; Saheki, T.; Tsuda, M.; Katsunuma, T.
    Studies of rat liver argininosuccinate synthetase. The presence of three forms, and their physicochemical, catalytic, and immunochemical properties (1979), J. Biochem., 86, 1353-1359.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.4.5 in presence of 5 mM argininosuccinate, the activities of the three forms of the enzyme are resistant to chymotrypsin Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.5 5,5'-dithiobis(2-nitrobenzoate)
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.5 0.022
-
 AMP citrulline, enzyme form ASSII Rattus norvegicus
6.3.4.5 0.026
-
 L-citrulline enzyme form ASSI Rattus norvegicus
6.3.4.5 0.037
-
 L-Asp enzyme form ASSII Rattus norvegicus
6.3.4.5 0.038
-
 L-Asp enzyme form ASSI Rattus norvegicus
6.3.4.5 0.11
-
 ATP enzyme form ASSI Rattus norvegicus
6.3.4.5 0.17
-
 ATP enzyme form ASSII Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.5 48000
-
 x * 48000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.5 3 enzyme forms ASSI, ASSII, and ASSIII Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.5 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-Asp
-
 Rattus norvegicus AMP + diphosphate + L-argininosuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.5 ? x * 48000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.5 45
-
 enzyme forms ASSI and II are stable, enzyme form ASSIII is very unstable Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.5 8.6
-
 reaction of ATP + citrulline + L-Asp, enzyme forms ASSI and ASSII Rattus norvegicus