BRENDA - Enzyme Database

Enzymes of arginine and urea synthesis

Ratner, S.; Adv. Enzymol. Relat. Areas Mol. Biol. 39, 1-90 (1973)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
6.3.4.5
-
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.5
AMP
competitive with respect to ATP
Bos taurus
6.3.4.5
D-Asp
-
Bos taurus
6.3.4.5
diphosphate
-
Bos taurus
6.3.4.5
erythro-3-Hydroxy-3-methylaspartate
-
Bos taurus
6.3.4.5
L-argininosuccinate
competitive with respect to citrulline, Asp, and ATP
Bos taurus
6.3.4.5
threo-3-Hydroxy-3-methylaspartate
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.5
0.035
-
L-Asp
-
Bos taurus
6.3.4.5
0.88
-
threo-beta-methyl-L-Asp
-
Bos taurus
6.3.4.5
4.5
-
threo-beta-hydroxy-L-Asp
-
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.1
30800
-
4 * 30800, sedimentation equilibrium urea
Rattus norvegicus
6.3.4.5
45000
-
4 * 45000, two pairs of monomers crosslinked by disulfide bonds, SDS-PAGE in presence of reducing agent
Bos taurus
6.3.4.5
90000
-
2 * 90000, SDS-PAGE in absence of reducing agent
Bos taurus
6.3.4.5
175000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.1
L-arginine + H2O
Rattus norvegicus
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.1
Rattus norvegicus
-
-
-
6.3.4.5
Bos taurus
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
6.3.4.5
ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate
mechanism
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.4.5
kidney
-
Bos taurus
-
6.3.4.5
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.1
additional information
-
-
Rattus norvegicus
6.3.4.5
3.83
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.1
L-arginine + H2O
-
1574
Rattus norvegicus
L-ornithine + urea
-
1574
Rattus norvegicus
?
3.5.3.1
L-arginine + H2O
-
1574
Rattus norvegicus
?
-
-
-
-
6.3.4.5
ATP + L-citrulline + L-Asp
r
1574
Bos taurus
AMP + diphosphate + L-argininosuccinate
-
1574
Bos taurus
-
6.3.4.5
ATP + L-citrulline + L-Asp
D-Asp is inactive as substrate
1574
Bos taurus
AMP + diphosphate + L-argininosuccinate
-
1574
Bos taurus
-
6.3.4.5
ATP + L-citrulline + threo-beta-hydroxy-DL-Asp
-
1574
Bos taurus
?
-
-
-
-
6.3.4.5
ATP + L-citrulline + threo-beta-methyl-L-Asp
-
1574
Bos taurus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.1
tetramer
4 * 30800, sedimentation equilibrium urea
Rattus norvegicus
6.3.4.5
tetramer
2 * 90000, SDS-PAGE in absence of reducing agent; 4 * 45000, two pairs of monomers crosslinked by disulfide bonds, SDS-PAGE in presence of reducing agent
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.4.5
6
-
reaction of AMP + diphosphate + L-argininosuccinate
Bos taurus
6.3.4.5
8.7
-
reaction of ATP + L-citulline + L-Asp
Bos taurus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
6.3.4.5
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.5
AMP
competitive with respect to ATP
Bos taurus
6.3.4.5
D-Asp
-
Bos taurus
6.3.4.5
diphosphate
-
Bos taurus
6.3.4.5
erythro-3-Hydroxy-3-methylaspartate
-
Bos taurus
6.3.4.5
L-argininosuccinate
competitive with respect to citrulline, Asp, and ATP
Bos taurus
6.3.4.5
threo-3-Hydroxy-3-methylaspartate
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.5
0.035
-
L-Asp
-
Bos taurus
6.3.4.5
0.88
-
threo-beta-methyl-L-Asp
-
Bos taurus
6.3.4.5
4.5
-
threo-beta-hydroxy-L-Asp
-
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.1
30800
-
4 * 30800, sedimentation equilibrium urea
Rattus norvegicus
6.3.4.5
45000
-
4 * 45000, two pairs of monomers crosslinked by disulfide bonds, SDS-PAGE in presence of reducing agent
Bos taurus
6.3.4.5
90000
-
2 * 90000, SDS-PAGE in absence of reducing agent
Bos taurus
6.3.4.5
175000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.1
L-arginine + H2O
Rattus norvegicus
-
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.4.5
kidney
-
Bos taurus
-
6.3.4.5
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.1
additional information
-
-
Rattus norvegicus
6.3.4.5
3.83
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.1
L-arginine + H2O
-
1574
Rattus norvegicus
L-ornithine + urea
-
1574
Rattus norvegicus
?
3.5.3.1
L-arginine + H2O
-
1574
Rattus norvegicus
?
-
-
-
-
6.3.4.5
ATP + L-citrulline + L-Asp
r
1574
Bos taurus
AMP + diphosphate + L-argininosuccinate
-
1574
Bos taurus
-
6.3.4.5
ATP + L-citrulline + L-Asp
D-Asp is inactive as substrate
1574
Bos taurus
AMP + diphosphate + L-argininosuccinate
-
1574
Bos taurus
-
6.3.4.5
ATP + L-citrulline + threo-beta-hydroxy-DL-Asp
-
1574
Bos taurus
?
-
-
-
-
6.3.4.5
ATP + L-citrulline + threo-beta-methyl-L-Asp
-
1574
Bos taurus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.1
tetramer
4 * 30800, sedimentation equilibrium urea
Rattus norvegicus
6.3.4.5
tetramer
2 * 90000, SDS-PAGE in absence of reducing agent; 4 * 45000, two pairs of monomers crosslinked by disulfide bonds, SDS-PAGE in presence of reducing agent
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.4.5
6
-
reaction of AMP + diphosphate + L-argininosuccinate
Bos taurus
6.3.4.5
8.7
-
reaction of ATP + L-citulline + L-Asp
Bos taurus