BRENDA - Enzyme Database

Human adenylosuccinate synthetase. Partial purification, kinetic and regulatory properties of the enzyme from placenta

Van der Weyden, M.B.; Kelly, W.N.; J. Biol. Chem. 249, 7282-7289 (1974)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.4
3',5'-cyclic GMP
-
Homo sapiens
6.3.4.4
6-mercaptopurine ribonucleotide
-
Homo sapiens
6.3.4.4
adenosine 3',5'-AMP
-
Homo sapiens
6.3.4.4
adenylosuccinate
competitive with respect to IMP
Homo sapiens
6.3.4.4
ADP
-
Homo sapiens
6.3.4.4
AMP
competitive with respect to IMP
Homo sapiens
6.3.4.4
CDP
-
Homo sapiens
6.3.4.4
CMP
-
Homo sapiens
6.3.4.4
dAMP
-
Homo sapiens
6.3.4.4
dCMP
-
Homo sapiens
6.3.4.4
dGDP
-
Homo sapiens
6.3.4.4
dGMP
-
Homo sapiens
6.3.4.4
GDP
competitive with respect to GTP
Homo sapiens
6.3.4.4
GMP
competitive with respect to GTP
Homo sapiens
6.3.4.4
IDP
-
Homo sapiens
6.3.4.4
succinate
-
Homo sapiens
6.3.4.4
TDP
-
Homo sapiens
6.3.4.4
TMP
-
Homo sapiens
6.3.4.4
UDP
-
Homo sapiens
6.3.4.4
UMP
-
Homo sapiens
6.3.4.4
XMP
competitive with respect to IMP
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.4
0.031
-
GTP
-
Homo sapiens
6.3.4.4
0.037
-
IMP
-
Homo sapiens
6.3.4.4
0.95
-
Asp
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.4.4
Ba2+
can replace Mg2+ with lower efficiency
Homo sapiens
6.3.4.4
Ca2+
can replace Mg2+ with lower efficiency
Homo sapiens
6.3.4.4
Co2+
can replace Mg2+ with lower efficiency
Homo sapiens
6.3.4.4
Mg2+
divalent cation required, maximal activity obtained with Mg2+
Homo sapiens
6.3.4.4
Mn2+
can replace Mg2+ with lower efficiency
Homo sapiens
6.3.4.4
Ni2+
can replace Mg2+ with lower efficiency
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.4.4
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.4.4
partial
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
6.3.4.4
GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP
sequential rapid equilibrium fully random mechanism
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.4.4
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.4.4
0.00641
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.4
GTP + IMP + L-Asp
-
1548
Homo sapiens
GDP + phosphate + adenylosuccinate
-
1548
Homo sapiens
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.4.4
6.5
7.4
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.4
3',5'-cyclic GMP
-
Homo sapiens
6.3.4.4
6-mercaptopurine ribonucleotide
-
Homo sapiens
6.3.4.4
adenosine 3',5'-AMP
-
Homo sapiens
6.3.4.4
adenylosuccinate
competitive with respect to IMP
Homo sapiens
6.3.4.4
ADP
-
Homo sapiens
6.3.4.4
AMP
competitive with respect to IMP
Homo sapiens
6.3.4.4
CDP
-
Homo sapiens
6.3.4.4
CMP
-
Homo sapiens
6.3.4.4
dAMP
-
Homo sapiens
6.3.4.4
dCMP
-
Homo sapiens
6.3.4.4
dGDP
-
Homo sapiens
6.3.4.4
dGMP
-
Homo sapiens
6.3.4.4
GDP
competitive with respect to GTP
Homo sapiens
6.3.4.4
GMP
competitive with respect to GTP
Homo sapiens
6.3.4.4
IDP
-
Homo sapiens
6.3.4.4
succinate
-
Homo sapiens
6.3.4.4
TDP
-
Homo sapiens
6.3.4.4
TMP
-
Homo sapiens
6.3.4.4
UDP
-
Homo sapiens
6.3.4.4
UMP
-
Homo sapiens
6.3.4.4
XMP
competitive with respect to IMP
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.4
0.031
-
GTP
-
Homo sapiens
6.3.4.4
0.037
-
IMP
-
Homo sapiens
6.3.4.4
0.95
-
Asp
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.4.4
Ba2+
can replace Mg2+ with lower efficiency
Homo sapiens
6.3.4.4
Ca2+
can replace Mg2+ with lower efficiency
Homo sapiens
6.3.4.4
Co2+
can replace Mg2+ with lower efficiency
Homo sapiens
6.3.4.4
Mg2+
divalent cation required, maximal activity obtained with Mg2+
Homo sapiens
6.3.4.4
Mn2+
can replace Mg2+ with lower efficiency
Homo sapiens
6.3.4.4
Ni2+
can replace Mg2+ with lower efficiency
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.4.4
partial
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.4.4
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.4.4
0.00641
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.4
GTP + IMP + L-Asp
-
1548
Homo sapiens
GDP + phosphate + adenylosuccinate
-
1548
Homo sapiens
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.4.4
6.5
7.4
-
Homo sapiens