EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.3 | H125S | site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion | Clostridium cylindrosporum |
6.3.4.3 | H131S | site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion | Clostridium cylindrosporum |
6.3.4.3 | H268S | site-directed mutant enzymes H125S, H131S, and H268S do not fold properly and/or do not associate to the active tetramer and, as a consequence are susceptible to intracellular proteolytic digestion | Clostridium cylindrosporum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.3 | 0.13 | - |
ADP | tetrahydrofolate, mutant enzyme K71Q | Clostridium cylindrosporum | |
6.3.4.3 | 0.23 | - |
tetrahydrofolate | MgATP2-, wild-type enzyme | Clostridium cylindrosporum | |
6.3.4.3 | 0.26 | - |
ATP | mutant enzyme K71Q | Clostridium cylindrosporum | |
6.3.4.3 | 0.4 | - |
ATP | mutant enzyme H131S | Clostridium cylindrosporum | |
6.3.4.3 | 0.59 | - |
ATP | mutant enzyme H125S | Clostridium cylindrosporum | |
6.3.4.3 | 0.69 | - |
tetrahydrofolate | wild-type enzyme | Clostridium cylindrosporum | |
6.3.4.3 | 0.97 | - |
tetrahydrofolate | mutant enzyme H125S | Clostridium cylindrosporum | |
6.3.4.3 | 1.12 | - |
tetrahydrofolate | mutant enzyme H131S | Clostridium cylindrosporum | |
6.3.4.3 | 6.7 | - |
formate | wild-type enzyme | Clostridium cylindrosporum | |
6.3.4.3 | 8.2 | - |
formate | mutant enzyme H125S | Clostridium cylindrosporum | |
6.3.4.3 | 12.1 | - |
formate | mutant enzyme K71Q | Clostridium cylindrosporum | |
6.3.4.3 | 20.2 | - |
formate | mutant enzyme H131S | Clostridium cylindrosporum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.3 | Clostridium cylindrosporum | - |
wild-type and site-directed mutant enzymes H125S, H131S, and H268S | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.3 | ATP + formate + tetrahydrofolate | - |
Clostridium cylindrosporum | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
6.3.4.3 | ATP + formate + tetrahydrofolate | ATP in form of MgATP2- | Clostridium cylindrosporum | ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.4.3 | additional information | - |
additional information | - |
Clostridium cylindrosporum |